Structure of PDB 7aud Chain F

Receptor sequence

>7audF (length=516) Species: 9606 (Homo sapiens)

KHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILM
PTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTG
DKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLL
ARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQK
DILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYD
SGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDG
CQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHC
LLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRI
QLLAYFGENGFNPDFCKKHPDVSCDNCCSKALVAKVSQREEMVKKCLGEL
TEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLE
KYGAEVISVLQKYSEW

3D structure

• PDB: 7aud Uncovering an allosteric mode of action for a selective inhibitor of human Bloom syndrome protein.
• Chain: F
• Resolution: 2.96 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	F	L716 R717 T738 G739 T766 E768 K769 S773 N774 R775 F807 R808 L896 S897 R898 H919 A920 T946 K968 E1008	L77 R78 T99 G100 T127 E129 K130 S134 N135 R136 F168 R169 L257 S258 R259 H280 A281 T307 K329 E369
BS02	ZN	F	C1036 C1055 C1063 C1066	C397 C416 C424 C427
BS03	ADP	F	L665 N667 R669 Q672 T691 G692 G693 G694 K695 S696	L26 N28 R30 Q33 T52 G53 G54 G55 K56 S57
BS04	RY8	F	H798 S801 Q802 H805 D840 E971 G972 Q975 T1015 T1018 N1022	H159 S162 Q163 H166 D201 E332 G333 Q336 T376 T379 N383

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006310 DNA recombination
• GO:0044237 cellular metabolic process

External links

• PDB: RCSB:7aud, PDBe:7aud, PDBj:7aud
• PDBsum: 7aud
• PubMed: 33647232
• UniProt: P54132|BLM_HUMAN RecQ-like DNA helicase BLM (Gene Name=BLM)