Structure of PDB 6waa Chain F

Receptor sequence

>6waaF (length=707) Species: 507522 (Klebsiella pneumoniae 342)

GKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTW
EVSSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHI
ATLLCALFVRHFRTLVKEGHVYVALPPLYRIDLGKEVYYALTEEEKTGVL
EQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLVISDEDEQQ
TTAIMDMLLAKKRSEDRRNWLQEKGDMAERLALHEFTENAYLNYSMYVIM
DRALPFIGDGLKPVQRRIVYAMSELGLNASAKFKKSARTVGDVLGKYHPH
GDSACYEAMVLMAQPFSYRYPLVDGQGNWGAPDDPKSFAAMRYTESRLSK
YAELLLSELGQGTVDWVPNFDGTLQEPKMLPARLPNILLNGTTGIAVGMA
TDIPPHNLREVAKAAITLIEQPKTTLDELLDIVQGPDFPTEAEIITSRAE
IRKIYQNGRGSVRMRAVWSKEDGAVVITALPHQVSGAKVLEQIAAQMRNK
KLPMVDDLRDESDHENPTRLVIVPRSNRVDMEQVMNHLFATTDLEKSYRI
NLNMIGLDGRPAVKNLLEILSEWLVFRRDTVRRRLNHRLEKVLKRLHILE
GLLVAFLNIDEVIEIIRTEDEPKPALMSRFGISETQAEAILELKLRHLAK
LEEMKIRGEQSELEKERDQLQAILASERKMNNLLKKELQADADAFGDDRR
SPLHERE

3D structure

• PDB: 6waa Topoisomerase Inhibitors Addressing Fluoroquinolone Resistance in Gram-Negative Bacteria.
• Chain: F
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	F	E419 G443 D495 R1029 K1039 V1041 H1075 H1077 G1078 T1170 I1172	E20 G44 D96 R252 K262 V264 H298 H300 G301 T393 I395
BS02	dna	F	L446 N447 S459 H498 S613 R616 R617 Y1018 Y1120 I1172 A1173 G1175 M1176 S1324 R1326	L47 N48 S60 H99 S214 R217 R218 Y241 Y343 I395 A396 G398 M399 S547 R549
BS03	MG	F	D491 D493	D92 D94
BS04	TNJ	F	K442 E461 S1080	K43 E62 S303

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:6waa, PDBe:6waa, PDBj:6waa
• PDBsum: 6waa
• PubMed: 32634310
• UniProt: B5XU60