Structure of PDB 6npf Chain F

Receptor sequence
>6npfF (length=434) Species: 562 (Escherichia coli) [Search protein sequence]
QQMGRGSMSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGAST
GSREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKI
MIDLDGTENKSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGK
YSMPVPMMNIINGGEDNDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVL
KAKGMNTAVGDEGGYAPNLGSNAEALAVIAEAVKAAGYELGKDITLAMDC
AASEFYKDGKYVLAGEGNKAFTSEEFTHFLEELTKQYPIVSIEDGLDESD
WDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIG
SLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSM
SRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQ
3D structure
PDB6npf Functional and structural basis of E. coli enolase inhibition by SF2312: a mimic of the carbanion intermediate.
ChainF
Resolution2.57 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S41 E167 E208 D245 E289 D316 K341 H369 K392
Catalytic site (residue number reindexed from 1) S49 E171 E212 D249 E293 D320 K345 H373 K396
Enzyme Commision number 4.2.1.11: phosphopyruvate hydratase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG F D245 E289 D316 D249 E293 D320
BS02 KVM F G39 A40 S41 Q166 E167 D245 E289 D316 K341 R370 S371 K392 G47 A48 S49 Q170 E171 D249 E293 D320 K345 R374 S375 K396
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004634 phosphopyruvate hydratase activity
GO:0005515 protein binding
GO:0016829 lyase activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0006096 glycolytic process
GO:0006396 RNA processing
GO:0006401 RNA catabolic process
Cellular Component
GO:0000015 phosphopyruvate hydratase complex
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0009986 cell surface
GO:0016020 membrane
GO:1990061 bacterial degradosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6npf, PDBe:6npf, PDBj:6npf
PDBsum6npf
PubMed31745118
UniProtP0A6P9|ENO_ECOLI Enolase (Gene Name=eno)

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