Structure of PDB 5wfs Chain F

Receptor sequence

>5wfsF (length=177) Species: 562 (Escherichia coli) [Search protein sequence]

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

PDB

5wfs Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.

Chain

Resolution

3.0 Å

3D
structure

[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]

Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	F	K32 T34 N36 M37 G38 G40 T67 A69 R70 F76 I78 I84 K87 S120 F121 D122 S128 G150 L151 D152 T154	K32 T34 N36 M37 G38 G40 T67 A69 R70 F76 I78 I84 K87 S120 F121 D122 S128 G150 L151 D152 T154
BS02	rna	F	S23 M25 Q26 Q62 K63 L65 T89 R91	S23 M25 Q26 Q62 K63 L65 T89 R91
BS03	rna	F	S72 V73 A74 R79	S72 V73 A74 R79

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome
GO:0008097	5S rRNA binding
GO:0019843	rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:5wfs, PDBe:5wfs, PDBj:5wfs
PDBsum	5wfs
PubMed	29733411
UniProt	P62399\|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)

[Back to BioLiP]