Structure of PDB 5utu Chain F

Receptor sequence

>5utuF (length=493) Species: 353152 (Cryptosporidium parvum Iowa II) [Search protein sequence]

MESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITGSL
HLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFAWK
NETIEDYWVCLNDAMTWRNPNDKDKICGPNLIVDDGGDATLILHEGVKAE
IEYEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGML
KDLYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCRQ
SLLHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDPI
CALQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYIA
NIGHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRLV
NLGCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSKE
LDEYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

PDB

5utu 2.65 Angstrom Resolution Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with SAH and NAD

Chain

Resolution

2.65 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
Catalytic site (residue number reindexed from 1)	H51 S74 S79 D135 E209 N234 K239 D243 N244 C248 H354 H408 S416 Q420
Enzyme Commision number	3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	NAD	F	K489 Y493	K487 Y491
BS02	NAD	F	T212 T213 T214 N246 C250 G277 E278 V279 E298 I299 T331 N333 I354 G355 N403 H410	T210 T211 T212 N244 C248 G275 E276 V277 E296 I297 T329 N331 I352 G353 N401 H408
BS03	SAH	F	L52 H53 T55 N78 D137 E211 T212 K241 D245 H356 F357 G409 H410 M415	L50 H51 T53 N76 D135 E209 T210 K239 D243 H354 F355 G407 H408 M413
BS04	ADN	F	L52 H53 T55 E57 D137 E211 T212 D245 L404 G409 H410 M415	L50 H51 T53 E55 D135 E209 T210 D243 L402 G407 H408 M413

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0004013	adenosylhomocysteinase activity
GO:0016787	hydrolase activity

	Biological Process
GO:0006730	one-carbon metabolic process
GO:0033353	S-adenosylmethionine cycle

	Cellular Component
GO:0005829	cytosol

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:5utu, PDBe:5utu, PDBj:5utu
PDBsum	5utu
PubMed
UniProt	Q5CPH1

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