Structure of PDB 5sc9 Chain F

Receptor sequence

>5sc9F (length=431) Species: 9606 (Homo sapiens)

DVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIA
TIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFA
GSPLSYRPVAIALDTKGPPGLSEQDVRDLRFGVEHGVDIVFASFVRKASD
VAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIE
IPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVAN
AVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRR
AAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAV
IAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLR
GFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 5sc9 Anthraquinone derivatives as ADP-competitive inhibitors of liver pyruvate kinase.
• Chain: F
• Resolution: 1.685 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	F	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 G532 Y533 T534	L331 T332 T333 T334 S337 W382 R389 G414 R416 P417 G418 S419 G420 Y421 T422
BS02	OXL	F	K282 E284 A305 G307 D308 T340	K170 E172 A193 G195 D196 T228
BS03	I7K	F	T62 P65 N87 H90 Y95 S374 G375 K379	T51 P54 N76 H79 Y84 S262 G263 K267
BS04	MG	F	E284 D308	E172 D196
BS05	MG	F	N87 S89 D125 T126	N76 S78 D114 T115

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:5sc9, PDBe:5sc9, PDBj:5sc9
• PDBsum: 5sc9
• PubMed: 35290845
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)