Structure of PDB 5sc8 Chain F

Receptor sequence

>5sc8F (length=432) Species: 9606 (Homo sapiens)

ADVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSII
ATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESF
AGSPLSYRPVAIALDTKGPPGLSEQDVRDLRFGVEHGVDIVFASFVRKAS
DVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGI
EIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVA
NAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELR
RAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAA
VIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKL
RGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 5sc8 Anthraquinone derivatives as ADP-competitive inhibitors of liver pyruvate kinase.
• Chain: F
• Resolution: 1.77 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	F	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 Y533 T534	L332 T333 T334 T335 S338 W383 R390 G415 R417 P418 G419 S420 Y422 T423
BS02	OXL	F	K282 E284 A305 G307 D308 T340	K171 E173 A194 G196 D197 T229
BS03	E6K	F	T62 P65 N87 H90 G91 Y95 S374 A378 K379	T52 P55 N77 H80 G81 Y85 S263 A267 K268
BS04	MG	F	E284 D308	E173 D197

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:5sc8, PDBe:5sc8, PDBj:5sc8
• PDBsum: 5sc8
• PubMed: 35290845
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)