Structure of PDB 5hxw Chain F

Receptor sequence

>5hxwF (length=430) Species: 585 (Proteus vulgaris)

GFVEGTEGALPKQADVVVVGAGILGIMTAINLVERGLSVVIVEKGNIAGE
QSSRFYGQAISYKMPDETFLLHHLGKHRWREMNAKVGIDTTYRTQGRVEV
PLDEEDLVNVRKWIDERSKNVGSDIPFKTRIIEGAELNQRLRGATTDWKI
AGFEEDSGSFDPEVATFVMAEYAKKMGVRIYTQCAARGLETQAGVISDVV
TEKGAIKTSQVVVAGGVWSRLFMQNLNVDVPTLPAYQSQQLISGSPTAPG
GNVALPGGIFFREQADGTYATSPRVIVAPVVKESFTYGYKYLPLLALPDF
PVHISLNEQLINSFMQSTHWNLDEVSPFEQFRNMTALPDLPELNASLEKL
KAEFPAFKESKLIDQWSGAMAIAPDENPIISEVKEYPGLVINTATGWGMT
ESPVSAELTADLLLGKKPVLDPKPFSLYRF

3D structure

• PDB: 5hxw Crystal structure of a membrane-bound l-amino acid deaminase from Proteus vulgaris
• Chain: F
• Resolution: 2.63 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): F96 Q99 A100 Q278 V322 F326
• Catalytic site (residue number reindexed from 1): F55 Q58 A59 Q237 V281 F285
• Enzyme Commision number: 1.4.3.2: L-amino-acid oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	F	V60 G61 G63 I64 L65 E84 K85 E91 Q92 S93 F96 Y97 Q99 A227 G256 G257 W259 Q280 F369 G409 G437 W438 G439 T441	V19 G20 G22 I23 L24 E43 K44 E50 Q51 S52 F55 Y56 Q58 A186 G215 G216 W218 Q239 F328 G368 G396 W397 G398 T400
BS02	16A	F	L347 L351 I352	L306 L310 I311
BS03	16A	F	G329 Y332 F341 I345 E417 W438	G288 Y291 F300 I304 E376 W397

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5hxw, PDBe:5hxw, PDBj:5hxw
• PDBsum: 5hxw
• PubMed: 27422658
• UniProt: Q9LCB2