Structure of PDB 5akn Chain F

Receptor sequence

>5aknF (length=184) [Search protein sequence]

ENVSGISAYLLGLIIGDGGLYKLKYKGNRSEYRVVITAKSENLIKQHIAP
LMQFLIDELNVKSKIQIVKGDTRYELRVSSKKLYYYFANMLERIRLFNMR
EQIAFIKGLYVAEGDMTLKRLRIWNKNKALLEIVSRWLNNLGVRNTIHLD
DHRHGVYVLNISLRDRIKFVHTILSSHLNPLPPE

3D structure

PDB

5akn Engineering a Nickase on the Homing Endonuclease I-Dmoi Scaffold.

Chain

Resolution

2.75 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	F	D21 T41 K43 T76 R77 R124 T150 H152 D154 H156 R157 S166 L167	D17 T37 K39 T72 R73 R120 T146 H148 D150 H152 R153 S162 L163
BS02	dna	F	G20 G22 Y25 Y29 N32 R33 S34 E35 Y36 R37 S67 K68 Q70 R77 R81 S83 S84 K85 E117 W128 N129 K130 D155 V160	G16 G18 Y21 Y25 N28 R29 S30 E31 Y32 R33 S63 K64 Q66 R73 R77 S79 S80 K81 E113 W124 N125 K126 D151 V156
BS03	dna	F	D21 A116 G118 D119 R126 W128	D17 A112 G114 D115 R122 W124
BS04	MN	F	D21 A116	D17 A112
BS05	MN	F	G20 E117	G16 E113

Gene Ontology

	Molecular Function
GO:0004519	endonuclease activity

	Biological Process
GO:0006314	intron homing
GO:0016539	intein-mediated protein splicing

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Molecular Function

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Biological Process

External links

PDB	RCSB:5akn, PDBe:5akn, PDBj:5akn
PDBsum	5akn
PubMed	26045557
UniProt	P21505\|DMO1_DESMO Homing endonuclease I-DmoI

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