Structure of PDB 5ahs Chain F

Receptor sequence

>5ahsF (length=391) Species: 1247726 (Advenella mimigardefordensis DPN7)

YELTPEQRTLQTQARELAQSVFASTAVQTDLTEQYPWDNVAQLRDAGFMG
MMLPTSVGGRGLSTLDTVIVIEEMAKACATMGRITVDSNLGAIGAITKYG
SEEQIKLAADLVLAGDKPAICISEPNAGSAASEMTTRADKNGDHYILNGE
KYWITGGGVSKLHLIFARVFDDGVEQGIGAFITVLDDHGPEGLKVGRRLY
AMGVRGIPETHLEFHDLKIHKSMMITFPDGLKRGFAALMSAYNAQRVGAG
AVALGIAQCAFEEGVAYLKRREQFGRPLAEFQGLQWMVADMSVQLEAARL
MLRSAAVSGETFPDINKAAQAKIFAAETANKVTNDALQFFGSSGYGRHNP
MERHVRDARMFTIAGGTAQILRTQVASKILDMKLPQTRDGY

3D structure

• PDB: 5ahs 3-Sulfinopropionyl-Coenzyme a (3Sp-Coa) Desulfinase from Advenella Mimigardefordensis Dpn7(T): Crystal Structure and Function of a Desulfinase with an Acyl-Coa Dehydrogenase Fold.
• Chain: F
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): I123 S124 Q246 G366 S378
• Catalytic site (residue number reindexed from 1): I122 S123 Q245 G365 S377
• Enzyme Commision number: 3.13.1.4: 3-sulfinopropanoyl-CoA desulfinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	F	Q283 W287	Q282 W286
BS02	FAD	F	R84 I121 I123 S124 G129 S130 W154 T156 E210 M361 A365 T368 Q370 I371 Q387	R83 I120 I122 S123 G128 S129 W153 T155 E209 M360 A364 T367 Q369 I370 Q386
BS03	COA	F	S130 A237 M240 S241 Y243 N244 R247 I316 G366 I371	S129 A236 M239 S240 Y242 N243 R246 I315 G365 I370

Gene Ontology

Molecular Function

• GO:0003995 acyl-CoA dehydrogenase activity
• GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
• GO:0016787 hydrolase activity
• GO:0050660 flavin adenine dinucleotide binding

External links

• PDB: RCSB:5ahs, PDBe:5ahs, PDBj:5ahs
• PDBsum: 5ahs
• PubMed: 26057676
• UniProt: K4L7X3|SPCAD_ADVMD 3-sulfinopropanoyl-CoA desulfinase (Gene Name=acd)