Structure of PDB 4m8d Chain F

Receptor sequence
>4m8dF (length=263) Species: 384765 (Roseibium aggregatum IAM 12614) [Search protein sequence]
MSAQSALSGLGAKLLSGEVEVVDCTGVLGPNTPILQLPPDFAKNTPKVEI
HKISEYDSDGPFFAWNWMVLGEHSGTHFDAPHHWITGKDYSDGFTDTLDV
QRLIAPVNVIDCSKESAADPDFLLTADLIKAWEAEHGEIGAGEWVVMRTD
WDKRAGDEAAFLNADETGPHSPGPTPDAIEYLLSKKIVGWGSQCIGTDAG
QAGGMEPPFPAHNLLHRDNCFGLASLANLDKLPAKGAILIAAPLKIERGT
GSPIRALALVPKA
3D structure
PDB4m8d A proton wire and water channel revealed in the crystal structure of isatin hydrolase.
ChainF
Resolution1.9 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.5.2.20: isatin hydrolase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 23J F F63 W65 F63 W65 MOAD: ic50~10uM
BS02 MN F H73 H77 D79 H83 H73 H77 D79 H83
BS03 23J F L35 H73 D79 H83 H212 L35 H73 D79 H83 H212 MOAD: ic50~10uM
Gene Ontology
Molecular Function
GO:0004061 arylformamidase activity
GO:0016787 hydrolase activity
GO:0016812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
Biological Process
GO:0019441 tryptophan catabolic process to kynurenine

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Molecular Function
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Biological Process
External links
PDB RCSB:4m8d, PDBe:4m8d, PDBj:4m8d
PDBsum4m8d
PubMed24917679
UniProtA0NLY7|ISAHY_ROSAI Isatin hydrolase (Gene Name=SIAM614_09648)

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