Structure of PDB 4k2s Chain F

Receptor sequence

>4k2sF (length=390) Species: 290398 (Chromohalobacter israelensis DSM 3043)

LKIRDAYTIVTCPGRNFVTLKIVTESGTHGIGDATLNGREMAVAAYLDEH
VVPALIGRDAGRIEDTWQYLYRGAYWRRGPVTMTAIAAVDMALWDIKAKA
AGMPLYQLLGGKSRERVMTYAHCTGQTIEDCLGEVARHVELGYRAVRVQS
GVPGIETTYGVSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVH
HRLTPIEAARLGKAVEPYHLFWLEDCVPAENQESLRLIREHTTTPLAIGE
VFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYHVRTGFH
GATDLSPVCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFEDGHF
LAGESPGHGVDIDEELAAKYPYERASLPVNRLEDGTLWHW

3D structure

• PDB: 4k2s Crystal structure of the mutant P317A of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and d-gluconate
• Chain: F
• Resolution: 1.699 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H124 R149 Q151 Y161 D213 H215 E239 G264 E265 R286 P288 H315 G316 E342 W405
• Catalytic site (residue number reindexed from 1): H122 R147 Q149 Y159 D198 H200 E224 G249 E250 R271 P273 H300 G301 E327 W390
• Enzyme Commision number: 4.2.1.-
  4.2.1.39: gluconate dehydratase.
  4.2.1.8: mannonate dehydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GCO	F	N39 Y161 D213 H215 E265 H315 A317 D319 E342 W405	N37 Y159 D198 H200 E250 H300 A302 D304 E327 W390
BS02	MG	F	D213 E239 E265	D198 E224 E250

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0008927 mannonate dehydratase activity
• GO:0016829 lyase activity
• GO:0046872 metal ion binding
• GO:0047929 gluconate dehydratase activity

Biological Process

• GO:0009063 amino acid catabolic process
• GO:0016052 carbohydrate catabolic process

External links

• PDB: RCSB:4k2s, PDBe:4k2s, PDBj:4k2s
• PDBsum: 4k2s
• UniProt: Q1QT89|DMGD_CHRSD D-galactonate dehydratase family member ManD (Gene Name=manD)