Structure of PDB 4ch8 Chain F

Receptor sequence

>4ch8F (length=253) Species: 9606 (Homo sapiens)

IVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPW
DKNFTENDLLVRIGKHSERNIEKISMLEKIYIHPRYNWRENLDRDIALMK
LKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGK
GQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGD
SGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVI
DQF

3D structure

• PDB: 4ch8 Gpibalpha Interacts Exclusively with Exosite II of Thrombin
• Chain: F
• Resolution: 1.75 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H363 D419 E522 G523 D524 S525 G526
• Catalytic site (residue number reindexed from 1): H43 D95 E198 G199 D200 S201 G202
• Enzyme Commision number: 3.4.21.5: thrombin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	F	E328 G330 M331 P333 W334 D433 H436 P437 C439 Y454 K455 R457 N484 M531 K532 P534 N537 R538 W539	E8 G10 M11 P13 W14 D109 H112 P113 C115 Y130 K131 R133 N160 M207 K208 P210 N213 R214 W215
BS02	peptide	F	R418 N504 F564 R565 K567 K568	R94 N180 F240 R241 K243 K244
BS03	0G6	F	H363 Y367 L416 D519 A520 S525 S546 W547 G548	H43 Y47 L92 D195 A196 S201 S222 W223 G224

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0005509 calcium ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0007596 blood coagulation

External links

• PDB: RCSB:4ch8, PDBe:4ch8, PDBj:4ch8
• PDBsum: 4ch8
• PubMed: 24316004
• UniProt: P00734|THRB_HUMAN Prothrombin (Gene Name=F2)