Structure of PDB 3wfr Chain F

Receptor sequence

>3wfrF (length=439) Species: 32630 (synthetic construct)

AAALNFYLSYFDDVAKVLPREHYCFIVGGWVRDRILGEPVGYNIDVDFLT
TADPVELAKNFAKRIGGHFFVFEPTIASVVLHLPPYRYRFDFSPLKGKDL
EKALIEDLKERDFTANAIAVNLDDVLTIVYDPTGGIKDLEQGLLRPVSIE
NLKRDPVRVLRGFRIAIEKNLQLTEDFYEFVKEDPRIVLKSAVERITHEL
FKIMKEKTAHKVIRELYEYGVLEAIIPEIGRLREVKDPLDEHTLKTLEYL
EQVIEDRAKYLSAELLENFGKKRVLGEFTDVELLKWGALFHDIGKPQTFA
VTFYEHDKVGAQIVREIGERLRWGDEATEFVAKLVRHHLRPFFLREAFKK
GELKRRGMANFWRECGDIAPHLFLLSIADAMASGDEEEDIKALMETIAEL
ESFNRNEMKAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

3D structure

• PDB: 3wfr Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase
• Chain: F
• Resolution: 3.501 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	F	D60 H81 F83 F85 I97 D112 R132 D133 N137 D182 R185 R188 E221 R222 L309 D364 X473 X477	D47 H68 F70 F72 I76 D91 R111 D112 N116 D155 R158 R161 E194 R195 L275 D325 X410 X414
BS02	CTP	F	G42 R45 D58 D60 R132 D133 N137 R185 R188	G29 R32 D45 D47 R111 D112 N116 R158 R161
BS03	POP	F	G42 R45 D58	G29 R32 D45
BS04	MG	F	D58 D60	D45 D47

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0016779 nucleotidyltransferase activity

Biological Process

• GO:0006396 RNA processing

External links

• PDB: RCSB:3wfr, PDBe:3wfr, PDBj:3wfr
• PDBsum: 3wfr
• PubMed: 24389024
• UniProt: O67911|CATNT_AQUAE CC-adding tRNA nucleotidyltransferase (Gene Name=aq_2158)