Structure of PDB 3u40 Chain F

Receptor sequence
>3u40F (length=227) Species: 5759 (Entamoeba histolytica) [Search protein sequence]
MAEHCPTPHNGAKYGEIAETVLMAGDPLRVKLLADTYLTDVVQYNSVRGA
VGYTGYYKGVKLSVQAHGMGMPSIGIYAYELFNFYGVKRIIRIGSAGAFD
ESLKLGDIVIGMGACYDSNFERQYDIPGKYSCIADFQLCREAVDAAEKLG
YRYKVGNIYSANYFYDDGDHSGAWKKMGVLAVEMEAAALYMIAARARKQA
LCMLTISDLCRRTKFTQMMEVALSLAK
3D structure
PDB3u40 Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.
ChainF
Resolution2.05 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H9 G25 R29 R48 E80 R92 S95 D208 C210 R222
Catalytic site (residue number reindexed from 1) H9 G25 R29 R48 E80 R92 S95 D208 C210 R211
Enzyme Commision number 2.4.2.1: purine-nucleoside phosphorylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADN F S95 F164 M184 E185 S95 F164 M184 E185
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004731 purine-nucleoside phosphorylase activity
GO:0004850 uridine phosphorylase activity
Biological Process
GO:0006139 nucleobase-containing compound metabolic process
GO:0006218 uridine catabolic process
GO:0009116 nucleoside metabolic process
Cellular Component
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:3u40, PDBe:3u40, PDBj:3u40
PDBsum3u40
PubMed21904041
UniProtC4LXG4

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