Structure of PDB 3t8w Chain F

Receptor sequence

>3t8wF (length=511) Species: 36329 (Plasmodium falciparum 3D7)

SEVPQVVSLDPTSIPIEYNTPIHDIKVQVYDIKGGCNVEEGLTIFLVNNP
GKENGPVKISSKVNDKQVSEFLKDENMEKFNVKLGTSKHFYMFNDNKNSV
AVGYVGCGSVADLSEADMKRVVLSLVTMLHDNKLSKLTVVFEINVDKNLF
RFFLETLFYEYMTDERFKSEYIKHLGVYINNADTYKEEVEKARVYYFGTY
YASQLIAAPSNYCNPVSLSNAAVELAQKLNLEYKILGVKELEELKMGAYL
SVGKGSMYPNKFIHLTYKSKGDVKKKIALVGKGITFDSGGYNLKAAPGSM
IDLMKFDMSGCAAVLGCAYCVGTLKPENVEIHFLSAVCENMVSKNSYRPG
DIITASNGKTIEVGNTDAEGRLTLADALVYAEKLGVDYIVDIATLTGAML
YSLGTSYAGVFGNNEELINKILQSSKTSNEPVWWLPIINEYRATLNSKYA
DINQISSSVKASSIVASLFLKEFVQNTAWAHIDIAGVSWNFKARKPKGFG
VRLLTEFVLND

3D structure

• PDB: 3t8w Bestatin-based chemical biology strategy reveals distinct roles for malaria M1- and M17-family aminopeptidases
• Chain: F
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K386 R463
• Catalytic site (residue number reindexed from 1): K294 R371
• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.13.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CO3	F	A460 G462 R463 L487	A368 G370 R371 L395
BS02	ZN	F	K374 D379 D399 E461	K282 D287 D307 E369
BS03	ZN	F	D379 D459 E461	D287 D367 E369
BS04	DGZ	F	K374 D379 A388 P389 M392 M396 F398 D399 N457 D459 E461 T486 L487 T488 G489 A490 Y493 I547 V551	K282 D287 A296 P297 M300 M304 F306 D307 N365 D367 E369 T394 L395 T396 G397 A398 Y401 I455 V459	MOAD: Ki=330nM PDBbind-CN: -logKd/Ki=7.54,Ki=29nM

Gene Ontology

Molecular Function

• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3t8w, PDBe:3t8w, PDBj:3t8w
• PDBsum: 3t8w
• PubMed: 21844374
• UniProt: Q8IL11|AMPL_PLAF7 Leucine aminopeptidase (Gene Name=LAP)