Structure of PDB 3nm3 Chain F

Receptor sequence
>3nm3F (length=507) Species: 5478 (Nakaseomyces glabratus) [Search protein sequence]
KFSKEQFDYSLYLVTDSGMIPEGKTLYGQVEAGLQNGVTLVQIREKDADT
KFFIEEALQIKELCHAHNVPLIINDRIDVAMAIGADGIHVGQDDMPIPMI
RKLVGPDMVIGWSVGFPEEVDELSKMGPDMVDYIGVGTLFPTLTKMGTAG
AIRVLDALERNNAHWCRTVGIGGLHPDNIERVLYQCVSSNGKRSLDGICV
VSDIIASLDAAKSTKILRGLIDKTDYKFVNIGLSTKNSLTTTDEIQSIIS
NTLKARPLVQHITNKVHQNFGANVTLALGSSPIMSEIQSEVNDLAAIPHA
TLLLNTGSVAPPEMLKAAIRAYNDVKRPIVFDPVGATETRLLLNNKLLTF
GQFSCIKGNSSEILGLAELSNELLIQATKIVAFKYKTVAVCTGEFDFIAD
GTIEGKYSLSKGTNGTSVEDIPCVAVEAGPIGCSLGSTIACMIGGQPSEG
NLFHAVVAGVMLYKAAGKIASEKCNGSGSFQVELIDALYRLTRENTPVTW
APKLTHT
3D structure
PDB3nm3 Domain Organization in Candida glabrata THI6, a Bifunctional Enzyme Required for Thiamin Biosynthesis in Eukaryotes .
ChainF
Resolution3.102 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) R45 S114 K146
Catalytic site (residue number reindexed from 1) R44 S113 K145
Enzyme Commision number 2.5.1.3: thiamine phosphate synthase.
2.7.1.50: hydroxyethylthiazole kinase.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 MG F D76 D95 D75 D94
BS02 TPS F Q43 R45 H90 S114 T143 T145 I179 G180 G181 V209 S210 Q42 R44 H89 S113 T142 T144 I171 G172 G173 V201 S202
BS03 POP F R45 N75 G92 S114 R44 N74 G91 S113
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004417 hydroxyethylthiazole kinase activity
GO:0004789 thiamine-phosphate diphosphorylase activity
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0046872 metal ion binding
Biological Process
GO:0009228 thiamine biosynthetic process
GO:0009229 thiamine diphosphate biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component
External links
PDB RCSB:3nm3, PDBe:3nm3, PDBj:3nm3
PDBsum3nm3
PubMed20968298
UniProtQ6FV03

[Back to BioLiP]