Structure of PDB 3hx0 Chain F

Receptor sequence

>3hx0F (length=329) Species: 9606 (Homo sapiens)

QKATNHNLHITEKLEVLAKAYSVQGDKWRAAGYAKAINALKSFHKPVTSY
QEACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGT
KTAQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIE
QTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSR
LLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPY
SEFACALLYFTGSAAFNASMRALAKTKGMSLSEHALSTAVVRNTHGAKVG
PGRVLPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 3hx0 Scrunching During DNA Repair Synthesis
• Chain: F
• Resolution: 3.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D181 D183 D244
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	F	W274 A277 S463 Q471 A514 R517 L527 S528 E529	W28 A31 S217 Q225 A268 R271 L281 S282 E283
BS02	dna	F	W342 G343 A344 G345 K347 T348 D490 Y505	W96 G97 A98 G99 K101 T102 D244 Y259
BS03	dna	F	Y267 W274 R275 G278 Y279 P303 G304 G306 R308 M309 K312	Y21 W28 R29 G32 Y33 P57 G58 G60 R62 M63 K66
BS04	dna	F	R389 T393 Y418	R143 T147 Y172
BS05	D3T	F	R386 G416 S417 R420 G426 D427 D429 Y505 F506 G508 A510 N513	R140 G170 S171 R174 G180 D181 D183 Y259 F260 G262 A264 N267
BS06	MG	F	D427 D429	D181 D183

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3hx0, PDBe:3hx0, PDBj:3hx0
• PDBsum: 3hx0
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)