Structure of PDB 3a6p Chain F

Receptor sequence

>3a6pF (length=1072) Species: 9606 (Homo sapiens)

AMDQVNALCEQLVKAVTVMMDPNSTQRYRLEALKFCEEFKEKCPICVPCG
LRLAEKTQVAIVRHFGLQILEHVVKFRWNGMSRLEKVYLKNSVMELIANG
TLNILEEENHIKDALSRIVVEMIKREWPQHWPDMLIELDTLSKQGETQTE
LVMFILLRLAEDVVTFQTLPPQRRRDIQQTLTQNMERIFSFLLNTLQENV
NKYQQVKTDTSQESKAQANCRVGVAALNTLAGYIDWVSMSHITAENCKLL
EILCLLLNEQELQLGAAECLLIAVSRKGKLEDRKPLMVLFGDVAMHYILS
AAQTADGGGLVEKHYVFLKRLCQVLCALGNQLCALLGADSDVETPSNFGK
YLESFLAFTTHPSQFLRSSTQMTWGALFRHEILSRDPLLLAIIPKYLRAS
MTNLVKMGFPSKTDSPSCEYSRFDFDSDEDFNAFFNSSRAQQGEVMRLAC
RLDPKTSFQMAGEWLKYQLSTFSLCSVFSPSFVQWEAMTLFLESVITQMF
RTLNREEIPVNDGIELLQMVLNFDTKDPLILSCVLTNVSALFPFVTYRPE
FLPQVFSKLFSSVTFETVEESKAPRTRAVRNVRRHACSSIIKMCRDYPQL
VLPNFDMLYNHVKQLLSNELLLTQMEKCALMEALVLISNQFKNYERQKVF
LEELMAPVASIWLSQDMHRVLSDVDAFIAYVGTDQKDPGLEDPCGLNRAR
MSFCVYSILGVVKRTCWPTDLEEAKAGGFVVGYTSSGNPIFRNPCTEQIL
KLLDNLLALIRTHNTLYAPEMLAKMAEPFTKALDMLDAEKSAILGLPQPL
LELNDSPVFKTVLERMQRFFSTLYENCFHILGKAGPSMQQDFYTVEDLAT
QLLSSAFVNLNNIPDYRLRPMLRVFVKPLVLFCPPEHYEALVSPILGPLF
TYLHMRLSQKWQVINQRESQEMLEEQLVRMLTREVMDLITVCCVSELTDL
GKCLMKHEDVCTALLITAFNSLAWKDTLSCQRTTSQLCWPLLKQVLSGTL
LADAVTWLFTSVLKGLQMHGQHDGCMASLVHLAFQIYEALRPRYLEIRAV
MEQIPEIQKDSLDQFDCKLLNP

3D structure

• PDB: 3a6p A high-resolution structure of the pre-microRNA nuclear export machinery
• Chain: F
• Resolution: 2.92 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	F	F1098 I1121	F1034 I1057
BS02	rna	F	S606 R734 D1087	S588 R714 D1023
BS03	rna	F	R602 T641 Q642 M643 G715 R718 L820 R835 R838 F839	R584 T623 Q624 M625 G695 R698 L800 R815 R818 F819

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0005049 nuclear export signal receptor activity
• GO:0005515 protein binding
• GO:0031267 small GTPase binding
• GO:0070883 pre-miRNA binding
• GO:1905172 RISC complex binding

Biological Process

• GO:0006405 RNA export from nucleus
• GO:0006611 protein export from nucleus
• GO:0006886 intracellular protein transport
• GO:0010586 miRNA metabolic process
• GO:0015031 protein transport
• GO:0031047 regulatory ncRNA-mediated gene silencing
• GO:0035281 pre-miRNA export from nucleus

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016442 RISC complex
• GO:0042565 RNA nuclear export complex

External links

• PDB: RCSB:3a6p, PDBe:3a6p, PDBj:3a6p
• PDBsum: 3a6p
• PubMed: 19965479
• UniProt: Q9HAV4|XPO5_HUMAN Exportin-5 (Gene Name=XPO5)