Structure of PDB 2ajq Chain F

Receptor sequence

>2ajqF (length=689) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSAIAANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGKRFGSHALEAWGYRLGEMKGEYKDDFKRMLEEQ
GEEYVDGMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTD
VGYTTFWSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARR
SELLRKLTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFKL
DTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKGAPVVD
DEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGK
IHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLDGIT
GKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQIAAEL
PTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAIAAL
RESIQQTLVESSQWVAGEQQVKWKRRWIKGLDGRKVHVRSPHAALNTLLQ
SAGALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEI
AQVVIETAQEAMRWVGDHWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

2ajq Structure of replicative DNA polymerase provides insigts into the mechanisms for processivity, frameshifting and editing

Chain

Resolution

2.6 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	F	T357 K359 V363 D365 D366 K394 R429 A438 Q439 P441 G442 H653	T342 K344 V348 D350 D351 K379 R414 A423 Q424 P426 G427 H638
BS02	dna	F	S337 S338 G402 K404 A425 V426 H432 N436 Q439 Y530 A532 G533 K536 W579 Q584 R604 S605 H607 N611 Q615	S322 S323 G387 K389 A410 V411 H417 N421 Q424 Y515 A517 G518 K521 W564 Q569 R589 S590 H592 N596 Q600

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:2ajq, PDBe:2ajq, PDBj:2ajq
PDBsum	2ajq
PubMed
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

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