Structure of PDB 1p8j Chain F

Receptor sequence

>1p8jF (length=468) Species: 10090 (Mus musculus)

DVYQEPTDPKFPQQWYLSGVTQRDLNVKEAWAQGFTGHGIVVSILDDGIE
KNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVAN
NGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPE
DDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDG
YTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLR
QKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLN
ADDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIVEILVE
PKDIGKRLEVRKAVTACLGEPNHITRLEHVQARLTLSYNRRGDLAIHLIS
PMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPAGEWVLEIENTSEA
NNYGTLTKFTLVLYGTAP

3D structure

• PDB: 1p8j The Crystal Structure of the Proprotein Processing Proteinase Furin Explains its Stringent Specificity
• Chain: F
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D153 H194
• Catalytic site (residue number reindexed from 1): D46 H87
• Enzyme Commision number: 3.4.21.75: furin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	F	Y495 Y560	Y388 Y453
BS02	peptide	F	D154 D191 N192 H194 E236 S253 W254 G255 P256 E257 D258 D264 G294 N295 D306 Y308 S368	D47 D84 N85 H87 E129 S146 W147 G148 P149 E150 D151 D157 G187 N188 D199 Y201 S261
BS03	peptide	F	H194 S368	H87 S261
BS04	CA	F	D115 D162 V205 N208 V210 G212	D8 D55 V98 N101 V103 G105
BS05	CA	F	D258 D301 E331	D151 D194 E224

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:1p8j, PDBe:1p8j, PDBj:1p8j
• PDBsum: 1p8j
• PubMed: 12794637
• UniProt: P23188|FURIN_MOUSE Furin (Gene Name=Furin)