Structure of PDB 1nu7 Chain F

Receptor sequence

>1nu7F (length=258) Species: 9606 (Homo sapiens)

IVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPW
DKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDI
ALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTA
NVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDA
CEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWI
QKVIDQFG

3D structure

• PDB: 1nu7 Staphylocoagulase is a prototype for the mechanism of cofactor-induced zymogen activation
• Chain: F
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C42 N98 M201 K202 S203 P204 N205
• Catalytic site (residue number reindexed from 1): C28 N95 M211 K212 S213 P214 N217
• Enzyme Commision number: 3.4.21.5: thrombin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	F	E23 G25 M26 P28 W29 F114 D116 H119 P120 C122 Y134 K135 R137 N159 M201 K202 N205 R206 W207	E8 G10 M11 P13 W14 F111 D113 H116 P117 C119 Y134 K135 R137 N164 M211 K212 N217 R218 W219
BS02	0ZJ	F	H57 Y60A W60D L99 I174 D189 A190 S195 S214 W215 G216 E217 G219	H43 Y47 W50 L96 I179 D199 A200 S205 S226 W227 G228 E229 G230

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0005509 calcium ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0007596 blood coagulation

External links

• PDB: RCSB:1nu7, PDBe:1nu7, PDBj:1nu7
• PDBsum: 1nu7
• PubMed: 14523451
• UniProt: P00734|THRB_HUMAN Prothrombin (Gene Name=F2)