Structure of PDB 1hr8 Chain F

Receptor sequence
>1hr8F (length=443) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
ASQIPGTRTSKLPNGLTIATEYIPNTSSATVGIFVDAGSRAENVKNNGTA
HFLQHLAFKGTQNRPQQGIELEIENIGSHLNAYTSRENTVYYAKSLQEDI
PKAVDILSDILTKSVLDNSAIERERDVIIRESEEVDKMYDEVVFDHLHEI
TYKDQPLGRTILGPIKNIKSITRTDLKDYITKNYKGDRMVLAGAGAVDHE
KLVQYAQKYFGHVPKSESPVPLGSPRGPLPVFCRGERFIKENTLPTTHIA
IALEGVSWSAPDYFVALATQAIVGNWDRAIGTGTNSPSPLAVAASQNGSL
ANSYMSFSTSYADSGLWGMYIVTDSNEHNVRLIVNEILKEWKRIKSGKIS
DAEVNRAKAQLKAALLLSLDGSTAIVEDIGRQVVTTGKRLSPEEVFEQVD
KITKDDIIMWANYRLQNKPVSMVALGNTSTVPNVSYIEEKLNQ
3D structure
PDB1hr8 Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences.
ChainF
Resolution2.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H70 Q73 H74 E143 E150
Catalytic site (residue number reindexed from 1) H51 Q54 H55 E124 E131
Enzyme Commision number 3.4.24.64: mitochondrial processing peptidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 peptide F Q73 F77 N100 A101 Y102 T103 V154 E160 I180 W277 L286 A290 S327 S329 Q379 L380 Q54 F58 N81 A82 Y83 T84 V135 E141 I161 W258 L267 A271 S308 S310 Q360 L361
BS02 ZN F H70 H74 E150 H51 H55 E131
Gene Ontology
Molecular Function
GO:0004222 metalloendopeptidase activity
GO:0005515 protein binding
GO:0008237 metallopeptidase activity
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006508 proteolysis
GO:0006627 protein processing involved in protein targeting to mitochondrion
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0017087 mitochondrial processing peptidase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1hr8, PDBe:1hr8, PDBj:1hr8
PDBsum1hr8
PubMed11470436
UniProtP10507|MPPB_YEAST Mitochondrial-processing peptidase subunit beta (Gene Name=MAS1)

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