Structure of PDB 7ug7 Chain EF

Receptor sequence

>7ug7EF (length=672) Species: 83333 (Escherichia coli K-12)

TTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKSAATTAFWSGMAKQ
YEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWR
QANKYKVPRIAFVNKMDRMGANFLKVVNQIKTRLGANPVPLQLAIGAEEH
FTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESAAE
ASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAM
LDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDP
FVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVR
AGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQ
EKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFN
VEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPG
SNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRL
HFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETPEENT
GDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRA
SYTMEFLKYDEAPSNVAQAVIE

3D structure

• PDB: 7ug7 The cyclic octapeptide antibiotic argyrin B inhibits translation by trapping EF-G on the ribosome during translocation.
• Chain: EF
• Resolution: 2.58 Å

Enzymatic activity

• Enzyme Commision number: 3.6.5.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	EF	P328 F329 V330 R359 R362 K507 S509 L591	P300 F301 V302 R331 R334 K479 S481 L563
BS02	rna	EF	H18 I19 H92 R146 K507 E625 T628 G629 G633 S636 R637 L642 K643 Q645 R671 S672 K675	H15 I16 H64 R118 K479 E597 T600 G601 G605 S608 R609 L614 K615 Q617 R643 S644 K647
BS03	rna	EF	Q508 G510 H584 V586 D587	Q480 G482 H556 V558 D559
BS04	rna	EF	G511 R512	G483 R484
BS05	peptide	EF	E413 P414 I416 S417 W448 D450 P486 Q487 V488 A489 L660 M682 F684	E385 P386 I388 S389 W420 D422 P458 Q459 V460 A461 L632 M654 F656
BS06	GDP	EF	D20 G22 K23 T24 T25 N142 K143 D145 S268 F270	D17 G19 K20 T21 T22 N114 K115 D117 S240 F242

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0032790 ribosome disassembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7ug7, PDBe:7ug7, PDBj:7ug7
• PDBsum: 7ug7
• PubMed: 35500116
• UniProt: P0A6M8|EFG_ECOLI Elongation factor G (Gene Name=fusA)