Structure of PDB 7n2v Chain EF

Receptor sequence

>7n2vEF (length=704) Species: 83333 (Escherichia coli K-12)

SARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATM
DWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVE
RSMRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGAN
FLKVVNQIKTRLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGV
TFEYEDIPADMVELANEWHQNLIESAAEASEELMEKYLGGEELTEAEIKG
ALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILD
DGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVL
NSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDP
DAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTD
VEGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYI
PAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIA
FKEGFKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVT
GVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIE
ARGK

3D structure

• PDB: 7n2v Structural basis of early translocation events on the ribosome.
• Chain: EF
• Resolution: 2.54 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	EF	H38 F329 V330 R359 R362 S509 R512 E548	H38 F329 V330 R359 R362 S509 R512 E548
BS02	rna	EF	H18 I19 H44 R59 H92 R146 G629 R637 L642 K643 G644 Q645 R671 S672 K675	H18 I19 H44 R59 H92 R146 G629 R637 L642 K643 G644 Q645 R671 S672 K675
BS03	rna	EF	K504 Q508 S509 G510 H584 V586	K504 Q508 S509 G510 H584 V586
BS04	GTP	EF	D20 G22 K23 T24 T25 V43 H44 T62 P90 G91 H92 N142 K143 D145 R146 F270	D20 G22 K23 T24 T25 V43 H44 T62 P90 G91 H92 N142 K143 D145 R146 F270
BS05	MG	EF	T24 T62	T24 T62

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0032790 ribosome disassembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7n2v, PDBe:7n2v, PDBj:7n2v
• PDBsum: 7n2v
• PubMed: 34234344
• UniProt: A0A0H3PU63