Structure of PDB 7n2c Chain EF

Receptor sequence

>7n2cEF (length=685) Species: 83333 (Escherichia coli K-12)

SARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHGITITS
AATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCA
VGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKTRLGAN
PVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELA
NEWHQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVT
CGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDE
PFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQ
MHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPV
ISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELH
LDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQY
GHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGP
LAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEP
IMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFG
YATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIE

3D structure

• PDB: 7n2c Structural basis of early translocation events on the ribosome.
• Chain: EF
• Resolution: 2.72 Å

Enzymatic activity

• Enzyme Commision number: 3.6.5.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	EF	R362 K541 G542 G543	R347 K526 G527 G528
BS02	rna	EF	G511 R512	G496 R497
BS03	rna	EF	H18 I19 H92 P121 R146 T628 G633 S636 R637 L642 K643 Q645 R671 S672 K675	H18 I19 H77 P106 R131 T613 G618 S621 R622 L627 K628 Q630 R656 S657 K660
BS04	rna	EF	Q508 S509 G510 H584 V586	Q493 S494 G495 H569 V571
BS05	FUA	EF	P90 F95 E98 D442 I468 I469 R472	P75 F80 E83 D427 I453 I454 R457
BS06	GDP	EF	D20 G22 K23 T24 T25 N142 K143 D145 S268 F270	D20 G22 K23 T24 T25 N127 K128 D130 S253 F255

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0032790 ribosome disassembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7n2c, PDBe:7n2c, PDBj:7n2c
• PDBsum: 7n2c
• PubMed: 34234344
• UniProt: P0A6M8|EFG_ECOLI Elongation factor G (Gene Name=fusA)