Structure of PDB 6z6b Chain EEE

Receptor sequence

>6z6bEEE (length=2031) Species: 11577 (La Crosse virus)

GMDYQEYQQFLARINTARDACVAKDIDVDLLMARHDYFGRELCKSLNIEY
RNDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSN
ESSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELY
PTIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCP
EFWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKK
DYSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLH
DQKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGK
MMDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQF
MINNDLIDKSEKLKLFKNFCGIGKHSKPKILDFDDANMYLASLTMMEQSK
KILSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDFS
TLMKNILSVSQYNRHNTFRIAMCANNNVFAIVFPSAATVVYSIIVLHKEE
ENIFNPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLFLTTCLLF
KHDNPTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLAISSNVKD
YIAEKFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYLSDYDITQ
KGIKDNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHHVMVDLAK
TILEIECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADTSRHNHLR
NRIENRNNFRRSITTISTFTSSKSCLKIGDFRKEKELQSVKQKKILEVQS
RKMRLANPMFNYEMLRNAMPNYTDYISTKVFDRLYELLDKKVLTDKPVIE
QIMDMMIDHKKFYFTFFNKGQKTSKDREIFVGEYEAKMCMYAVERIAKER
CKLNPDEMISEPGDGKLKVLEQKSEQEIRFLVETTRQKNREIDEAIEALA
SNLGKIEKLSLGKAKGLKMEINADMSKWSAQDVFYKYFWLIALDPILYPQ
EKERILYFMCNYMDKELILPDELLFNLLDQKVAYQNDIIATMTNQLNSNT
VLIKRNWLQGNFNYTSSYVHSCAMSVYKEILKEAITLLDGSILVNSLVHS
DDNQTSITIVQDKMENDKIIDFAMKEFERACLTFGCQANMKKTYVTNCIK
EFVSLFNLYGEPFSIYGRFLLTSVGDCAYIGPYEDLASRISSAQTAIKHG
CPPSLAWVSIAISHWMTSLTYNMLPGQSNDPIDYFPAENRKDIPIELNGV
LDAPLSMISTVGLESGNLYFLIKLLSKYTPVMQKRESVVNQIAEVKNWKV
EDLTDNEIFRLKILRYLVLDAGRSILTPRKFTTAGSLRKLYSFSKYQDRL
SSPGGMVELFTYLLEKPELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQ
NPAQLFIEQILFSHKPVIDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLE
LTNDDIQVIYSYIILNDPMMITIANTHILSIYGSPQRRMGMSCSTMPEFR
SPALVLRAYSKNNPDTEMARDLVHLKEFVENTNLEEKMKVRIAMNEAEKG
QRDIVFELKEMTRFYQVCYEYVKSTEHKIKVFILPAKSYTTTDFCSLMQG
NLIKDKEWYTVHYLKQILSGGHKAINATSEQNIAFECFKLITHFADSFID
SLSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLFRTGDLRQ
ADLDKYDAMKRILIRSLDYLNNDIFSLSRIKVGLDEFATIKKAHFSKMVS
FEGPPIKTGLLDLTELMKSQDLLNLNYDNIRNSNLISFSKLICCEGSDNI
NDGLEFLSDDPMNFTEGEAIHSTPIFNIYYSKRGERHMTYRNAIKLLIER
ETKIFEEAFTFSENGFISPENLGCLEAVVSLIKLLKTNEWSTVIDKCIHI
CLIKNGMDHMYHSFDVPKCFMGNPITRDINWVMFREFINSLPGTDIPPWN
VMTENFKKKCIALINSKFETQRDFSEFTKLM

3D structure

• PDB: 6z6b Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
• Chain: EEE
• Resolution: 3.961 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	EEE	Q291 K302 H306 C419 G420 K423 H424 P440 R592 A593 R595 R600 T642 K643 Y677 K679 E758 H760 H761 V764 K768 K1117 V1118 Y1120 D1123	Q292 K303 H307 C420 G421 K424 H425 P428 R574 A575 R577 R582 T624 K625 Y659 K661 E740 H742 H743 V746 K750 K1081 V1082 Y1084 D1087
BS02	rna	EEE	H312 N318 A320 K368 A371 R372 Q377 I378 N380 K381 K382 W395 Q397 Q398 L471 K472 Q506 N517 V521 Y524 R526 R531 M534 C535 A536 N537 N538 K859 K870 Q1313 S1314 I1511 L1512 F1513 H1515	H313 N319 A321 K369 A372 R373 Q378 I379 N381 K382 K383 W396 Q398 Q399 L459 K460 Q494 N505 V509 Y512 R514 R519 M522 C523 A524 N525 N526 K841 K852 Q1277 S1278 I1460 L1461 F1462 H1464
BS03	rna	EEE	N380 Q867	N381 Q849
BS04	ZN	EEE	C2064 H2169 I2173 D2178	C1844 H1949 I1953 D1958

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:6z6b, PDBe:6z6b, PDBj:6z6b
• PDBsum: 6z6b
• PubMed: 32681014
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)