Structure of PDB 8wwv Chain E

Receptor sequence

>8wwvE (length=489) Species: 1615674 (Pseudomonas lactis)

DFITKNNLWTNEQRDAADKVLAEIDSLGLEMIRLSWADQYGLLRGKSLTV
ASLKSAFKEGSEVAIGPFFFDLVSSMVFNLFTTAGFEDELSGNPTVVMVP
DPTTFKVLPWADKTGWMLADLHWKSGEPFPLCPRGIMKKAVKSLSDEGYL
FKCGIELEWYLTKIVDRSLSPESLGAPGVQPDAIQVQPVAQGYSVLLEHH
LDQVDDIMSKVRKGLLELNLPLRSIEDEWAPSQMETTFDVMEGLEAADAA
LLIKSAIKQICSRHGYHATFMCKPAINGFFASGWHMHQSLVDKDTRKNLF
IPSEGEVLSPLGRAYAGGLLANGSAASSFTTPTVNGYRRRQPYSLAPDRR
AWAKDNKAAMVRVVSATGDPASRIENRIGEPGANPYLYMASQIVSGLDGI
KNKKDPGELQESPYDAQVPMLPTTLAEALDALEHDSELFRSCFGDTFIKY
WLQLRRSEWARFLDAEGAEAAEPTGAVTQWEQKEYFNLL

3D structure

• PDB: 8wwv Discovery of the 1-naphthylamine biodegradation pathway reveals a glutamine synthetase-like protein that catalyzes 1-naphthylamine glutamylation
• Chain: E
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 6.3.1.2: glutamine synthetase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	E	G160 E162 R229 F244 D245 V246 H293 S295 K363 R368 R379 E381	G154 E156 R223 F238 D239 V240 H287 S289 K357 R362 R373 E375
BS02	P3S	E	E162 E164 E234 A287 H291 R345 L351 K363 R383	E156 E158 E228 A281 H285 R339 L345 K357 R377
BS03	MN	E	E164 E234 E241	E158 E228 E235
BS04	MN	E	E162 H291 E381	E156 H285 E375
BS05	MG	E	E162 E241	E156 E235

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004356 glutamine synthetase activity
• GO:0016879 ligase activity, forming carbon-nitrogen bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0006542 glutamine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0016020 membrane

External links

• PDB: RCSB:8wwv, PDBe:8wwv, PDBj:8wwv
• PDBsum: 8wwv
• UniProt: A0A7Y1Q2L1