Structure of PDB 8gri Chain E

Receptor sequence

>8griE (length=481) Species: 285500 (Streptomyces luteocolor)

TDVIVVGNGVLGLSVGVEIARTRPDVRVTLLGKPARQYGATPAAGAMLGA
FGEVTAHALASEHGRKKHALAVQAQRLWPEWIESLEATGTAADGRIKTAD
DTVVLLNTVGHSALDDANFAAVLTALKEANAPHEEIAVESVDWIDPDPNS
RPLRALHIEGEGSVDSGILLAALERSFLQAGGRLHPVDATEIRASHGRVE
GVVTDDGDFLPAGHVVVAAGARSQRLVAALPGLAHRIPRIYDGVGVSALV
DTWDGSGPATVLRTSNRAFACGLHLVPRAGGSVYIGATNAVCLEPRGAAS
IAETVFLFNCATHQLHRGLNGSELRKVQVGSRPAPIDGFPLIGGTSVEGL
WMLSGTYRDGLHMSPLLARHVVSLMDGGTGVDGLREFRPERDLISAWSRE
EILDDVVRHTMATGYEFPWRLPLEWPHMMETFLQGPFAELADRLSDTYTP
PADLMTAIMFSEREQQDELIAYYADVHREWH

3D structure

• PDB: 8gri N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry.
• Chain: E
• Resolution: 2.365 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	E	V16 G17 N18 G19 V20 L21 G42 R46 Q47 A50 T51 A54 M57 D198 A199 A229 G230 R232 G255 G340 R342 T366 Y367 R368 D369 G370 L371 H372	V6 G7 N8 G9 V10 L11 G32 R36 Q37 A40 T41 A44 M47 D188 A189 A219 G220 R222 G245 G330 R332 T356 Y357 R358 D359 G360 L361 H362
BS02	GLY	E	R342 R368	R332 R358
BS03	I55	E	N299 S310 T423 T466 F470	N289 S300 T413 T456 F460

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8gri, PDBe:8gri, PDBj:8gri
• PDBsum: 8gri
• PubMed: 37137912
• UniProt: A0A125SZC1