Structure of PDB 8eyk Chain E

Receptor sequence

>8eykE (length=966) Species: 9606 (Homo sapiens)

SAAIYNIDTSSESPDHYLVDHTLDGRVLFPATGYLSIVWKTLARALGLGV
EQLPVVFEDVVLHQATILPKTGTVSLEVRLLEASRAFEVSENGNLVVSGK
VYQWDDPDPRLFDHPESPTPNPTEPLFLAQAEVYKELRLRGYDYGPHFQG
ILEASLEGDSGRLLWKDNWVSFMDTMLQMSILGSAKHGLYLPTRVTAIHI
DPATHRQKLYTLQDKAQVADVVVSRWLRVTVAGGVHISGLHTESAPRRQQ
EQQVPILEKFCFTPHDSPIFLPVDDTSFRWVESLKGILADEDSSRPVWLK
AINCATSGVVGLVNCLRREPGGNRLRCVLLSNLSSTSHVPEVDPGSAELQ
KVLQGDLVMNVYRDGAWGAFRHFLLEEDKPEEPTAHAFVSTLTRGDLSSI
RWVCSSLRHAQPTCPGAQLCTVYYASLNFRDIMLATGKLSPDAIPGKWTS
QDSLLGMEFSGRDASGKRVMGLVPAKGLATSVLLSPDFLWDVPSNWTLEE
AASVPVVYSTAYYALVVRGRVRPGETLLIHSGSGGVGQAAIAIALSLGCR
VFTTVGSAEKRAYLQARFPQLDSTSFANSRDTSFEQHVLWHTGGKGVDLV
LNSLAEEKLQASVRCLATHGRFLEIGKFDLSQNHPLGMAIFLKNVTFHGV
LLDAFFNESSADWREVWALVQAGIRDGVVRPLKCTVFHGAQVEDAFRYMA
QGKHIGKVVVQVLAEEPEAVLKGAKPKLMSAISKTFCPAHKSYIIAGGLG
GFGLELAQWLIQRGVQKLVLTSRSGIRTGYQAKQVRRWRRQGVQVQVSTS
NISSLEGARGLIAEAAQLGPVGGVFNLAVVLRDGLLENQTPEFFQDVCKP
KYSGTLNLDRVTREACPELDYFVVFSSVSCGRGNAGQSNYGFANSAMERI
CEKRRHEGLPGLAVQWGAIGDVGILVETMSDTIVSGTLPQRMASCLEVLD
LFLNQPHMVLSSFVLA

3D structure

• PDB: 8eyk Atomic model for core modifying region of human fatty acid synthase in complex with Denifanstat.
• Chain: E
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 1.1.1.100: 3-oxoacyl-[acyl-carrier-protein] reductase.
  1.3.1.39: enoyl-[acyl-carrier-protein] reductase (NADPH, Re-specific).
  2.3.1.38: [acyl-carrier-protein] S-acetyltransferase.
  2.3.1.39: [acyl-carrier-protein] S-malonyltransferase.
  2.3.1.41: beta-ketoacyl-[acyl-carrier-protein] synthase I.
  2.3.1.85: fatty-acid synthase system.
  3.1.2.14: oleoyl-[acyl-carrier-protein] hydrolase.
  4.2.1.59: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	X5O	E	D1977 V2022 S2023 R2026 G2027 N2028 Q2031 Y2034 I2068 L2069 T2072 S2081 T2083	D833 V878 S879 R882 G883 N884 Q887 Y890 I924 L925 T928 S935 T937
BS02	NDP	E	F1573 T1654 S1675 S1677 G1678 G1679 V1680 G1700 K1704 S1723 R1724 I1769 V1794 L1795 L1796 A1844 Q1845 G1846	F429 T510 S531 S533 G534 G535 V536 G556 K560 S579 R580 I625 V650 L651 L652 A700 Q701 G702
BS03	NDP	E	G1891 G1894 G1895 F1896 S1916 R1917 N1945 L1971 A1972 V1973 K1995 Y2034 W2060 I2063 G2067 I2068	G747 G750 G751 F752 S772 R773 N801 L827 A828 V829 K851 Y890 W916 I919 G923 I924

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0031177 phosphopantetheine binding

Biological Process

• GO:0009058 biosynthetic process

External links

• PDB: RCSB:8eyk, PDBe:8eyk, PDBj:8eyk
• PDBsum: 8eyk
• PubMed: 37308485
• UniProt: P49327|FAS_HUMAN Fatty acid synthase (Gene Name=FASN)