Structure of PDB 8dh5 Chain E

Receptor sequence

>8dh5E (length=803) Species: 10760 (Escherichia phage T7) [Search protein sequence]

TINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFR
KMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFIKPEAVAYITIKT
TLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLN
KRVGHVYKKAFMQVVEADMLSKGLDSIHVGVRCIEMLIESTETIELAPEY
AEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTH
SKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVE
DIPAIWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMD
WRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVD
KVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGV
QHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIY
GIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWL
AYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPN
QAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRK
RCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRDSEIDAHKQESGIAP
NFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRE
TMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDILESDF
AFA

3D structure

PDB

8dh5 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.

Chain

Resolution

2.85 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	R50 R57 E63 R298 R423 Y427 T636 Y639 G640 S641 F644 G645 Y739 H772 P780	R48 R55 E61 R248 R352 Y356 T565 Y568 G569 S570 F573 G574 Y668 H692 P700
BS02	rna	E	N171 R386 R394 Y639 L752 D812	N150 R315 R323 Y568 L681 D732
BS03	dna	E	K679 K704	K608 K633
BS04	ATP	E	K472 Y571 R627 K631 M635 Y639	K401 Y500 R556 K560 M564 Y568

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0016779	nucleotidyltransferase activity
GO:0034062	5'-3' RNA polymerase activity

	Biological Process
GO:0006351	DNA-templated transcription
GO:0019083	viral transcription
GO:0039695	DNA-templated viral transcription

	Cellular Component
GO:0000428	DNA-directed RNA polymerase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8dh5, PDBe:8dh5, PDBj:8dh5
PDBsum	8dh5
PubMed	36635281
UniProt	P00573\|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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