Structure of PDB 8dh4 Chain E

Receptor sequence

>8dh4E (length=819) Species: 10760 (Escherichia phage T7) [Search protein sequence]

INIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRK
MFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVAFQFLQEIKPE
AVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHF
KKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHV
GVRCIEMLIESTLAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITG
GGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKK
VLAVANVITKWKHCPVEDIPAIAWKRAAAAVYRKDKARKSRRISLEFMLE
QANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIG
KEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWA
EQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDE
VGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTG
EISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLED
TIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAA
KLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLG
QDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSF
GTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPAL
PAKGNLNLRDILESDFAFA

3D structure

PDB

8dh4 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.

Chain

Resolution

2.8 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	R50 R57 E63 K164 R298 R423 Y427 T636 Y639 S641 F644 Y739 H772 P780	R47 R54 E60 K152 R265 R370 Y374 T583 Y586 S588 F591 Y686 H708 P716
BS02	rna	E	N171 R394 R425 Y639 I810 D812	N159 R341 R372 Y586 I746 D748
BS03	dna	E	E643 K679 K704	E590 K626 K651
BS04	S96	E	Y571 R627 K631 M635 Y639	Y518 R574 K578 M582 Y586

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0016779	nucleotidyltransferase activity
GO:0034062	5'-3' RNA polymerase activity

	Biological Process
GO:0006351	DNA-templated transcription
GO:0019083	viral transcription
GO:0039695	DNA-templated viral transcription

	Cellular Component
GO:0000428	DNA-directed RNA polymerase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8dh4, PDBe:8dh4, PDBj:8dh4
PDBsum	8dh4
PubMed	36635281
UniProt	P00573\|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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