Structure of PDB 8dh3 Chain E

Receptor sequence

>8dh3E (length=825) Species: 10760 (Escherichia phage T7) [Search protein sequence]

INIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRK
MFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEPTAFQFLQEIKPE
AVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHF
KKNVEEQLNKRVGHVYKKAFMQVVEADMLSSWHKEDSIHVGVRCIEMLIE
STGMVSLHETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITG
GGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKK
VLAVANVITKWKHCPVEDIPAIEALTAWKRAAAAVYRKDKARKSRRISLE
FMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKG
KPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLEN
TWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAM
LRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEVVTVTD
ENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQ
VLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWL
KSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNL
MFLGQFRDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFA
LIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQL
DKMPALPAKGNLNLRDILESDFAFA

3D structure

PDB

8dh3 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.

Chain

Resolution

3.0 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	R50 R57 E63 R298 R423 Y427 M431 T636 Y639 G640 S641 F644 Q649 K713 Y739 H772 P780 H784	R47 R54 E60 R265 R374 Y378 M382 T587 Y590 G591 S592 F595 Q600 K664 Y690 H714 P722 H726
BS02	rna	E	N171 K172 R386 K389 A390 R394 R425 Q435 G436 N437 Y639 I810 D812	N159 K160 R337 K340 A341 R345 R376 Q386 G387 N388 Y590 I752 D754

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0016779	nucleotidyltransferase activity
GO:0034062	5'-3' RNA polymerase activity

	Biological Process
GO:0006351	DNA-templated transcription
GO:0019083	viral transcription
GO:0039695	DNA-templated viral transcription

	Cellular Component
GO:0000428	DNA-directed RNA polymerase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8dh3, PDBe:8dh3, PDBj:8dh3
PDBsum	8dh3
PubMed	36635281
UniProt	P00573\|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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