Structure of PDB 8dh2 Chain E

Receptor sequence

>8dh2E (length=841) Species: 10760 (Escherichia phage T7) [Search protein sequence]

INIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRK
MFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAF
QFLQEIKPEAVAYITIKTTLACLTTTVQAVASAIGRAIEDEARFGRIRDL
EAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKE
DSIHVGVRCIEMLIESTGMVSLHRQNASETIELAPEYAEAIATRAGALAG
ISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYM
PEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIAWKRAAAA
VYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFN
PQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIE
ENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLP
LAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQ
ADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRS
VMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWE
SVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGF
PVWQEYKKPIQTRLNLMFLGQFRDSEIDAHKQESGIAPNFVHSQDGSHLR
KTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVL
ADFYDQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA

3D structure

PDB

8dh2 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.

Chain

Resolution

2.9 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	R50 R57 E63 R423 Y427 R632 T636 Y639 G640 S641 F644 Q648 Q649 E652 Y739 H772 P780	R47 R54 E60 R390 Y394 R599 T603 Y606 G607 S608 F611 Q615 Q616 E619 Y706 H730 P738
BS02	rna	E	N171 K172 K389 R394 R425 G436 I810 D812	N164 K165 K356 R361 R392 G403 I768 D770
BS03	dna	E	E643 E775	E610 E733
BS04	3PO	E	K472 R627 K631 M635	K439 R594 K598 M602

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0016779	nucleotidyltransferase activity
GO:0034062	5'-3' RNA polymerase activity

	Biological Process
GO:0006351	DNA-templated transcription
GO:0019083	viral transcription
GO:0039695	DNA-templated viral transcription

	Cellular Component
GO:0000428	DNA-directed RNA polymerase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8dh2, PDBe:8dh2, PDBj:8dh2
PDBsum	8dh2
PubMed	36635281
UniProt	P00573\|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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