Structure of PDB 8a8w Chain E

Receptor sequence

>8a8wE (length=569) Species: 1773 (Mycobacterium tuberculosis)

SLVLDQFGRNLTAAAMEGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGE
PGVGKTAVVEGLAQAIVHGEVPETLKDKQLYTLDLGSLVAGSRYRGDFEE
RLKKVLKEINTRGDIILFIDELHTLVGAIDAASILKPKLARGELQTIGAT
TLDEYRKYIEKDAALERRFQPVQVGEPTVEHTIEILKGLRDRYEAHHRVS
ITDAAMVAAATLADRYINDRFLPDKAIDLIDEAGARMRIRRMVAEVDDEQ
IAEVLGNWTGIPVFKLTEAETTRLLRMEEELHKRIIGQEDAVKAVSKAIR
RTRAGLKDPKRPSGSFIFAGPSGVGKTELSKALANFLFGDDDALIQIDMG
EFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLFDEIEKAHQE
IYNSLLQVLEDGRLTDGQGRTVDFKNTVLIFTSNLGTYERMKQKVNDELK
KHFRPEFLNRIDDIIVFHQLTREEIIRMVDLMISRVAGQLKSKDMALVLT
DAAKALLAKRGFDPVLGARPLRRTIQREIEDQLSEKILFEEVGPGQVVTV
DVDNWDGEGPGEDAVFTFT

3D structure

• PDB: 8a8w Structure of the drug target ClpC1 unfoldase in action provides insights on antibiotic mechanism of action.
• Chain: E
• Resolution: 4.29 Å

Enzymatic activity

• Enzyme Commision number: 3.4.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	E	R260 Y261 F589 G600 Y601 V602	R93 Y94 F356 G367 Y368 V369
BS02	ADP	E	P189 V190 I191 G219 G221 K222 T223 A224 I358	P22 V23 I24 G52 G54 K55 T56 A57 I185
BS03	ADP	E	R517 I518 I519 S555 G556 V557 G558 K559 T560 E561 M730 R771 R774	R284 I285 I286 S322 G323 V324 G325 K326 T327 E328 M478 R519 R522

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042803 protein homodimerization activity
• GO:0044183 protein folding chaperone

Biological Process

• GO:0006457 protein folding

Cellular Component

• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:8a8w, PDBe:8a8w, PDBj:8a8w
• PDBsum: 8a8w
• PubMed: 36208775
• UniProt: P9WPC9|CLPC1_MYCTU ATP-dependent Clp protease ATP-binding subunit ClpC1 (Gene Name=clpC1)