Structure of PDB 8a6w Chain E

Receptor sequence
>8a6wE (length=395) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]
APDVDLADGNFYASREARAAYRWMRANQPVFRDRNGLAAASTYQAVIDAE
RQPELFSNAGGIRPDQPALPMMIDMDDPAHLLRRKLVNAGFTRKRVKDKE
ASIAALCDTLIDAVCERGECDFVRDLAAPLPMAVIGDMLGVRPEQRDMFL
RWSDDLVTFLSSHVSQEDFQITMDAFAAYNDFTRATIAARRADPTDDLVS
VLVSSEVDGERLSDDELVMETLLILIGGDETTRHTLSGGTEQLLRNRDQW
DLLQRDPSLLPGAIEEMLRWTAPVKNMCRVLTADTEFHGTALCAGEKMML
LFESANFDEAVFCEPEKFDVQRNPNSHLAFGFGTHFCLGNQLARLELSLM
TERVLRRLPDLRLVADDSVLPLRPANFVSGLESMPVVFTPSPPLG
3D structure
PDB8a6w Towards automated crystallographic structure refinement with phenix.refine.
ChainE
Resolution2.09 Å
3D
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Enzymatic activity
Enzyme Commision number 1.14.15.28: cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming].
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 K2B E M176 G230 F380 M173 G227 F377
BS02 HEM E E53 I76 H83 R87 F94 I138 G230 G231 T234 T235 V277 R282 A332 F333 G334 F335 H338 C340 L341 G342 E50 I73 H80 R84 F91 I135 G227 G228 T231 T232 V274 R279 A329 F330 G331 F332 H335 C337 L338 G339
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008395 steroid hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0031073 cholesterol 26-hydroxylase activity
GO:0036199 cholest-4-en-3-one 26-monooxygenase activity
GO:0046872 metal ion binding
Biological Process
GO:0006707 cholesterol catabolic process
GO:0008203 cholesterol metabolic process
GO:0016042 lipid catabolic process
Cellular Component
GO:0009274 peptidoglycan-based cell wall

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8a6w, PDBe:8a6w, PDBj:8a6w
PDBsum8a6w
PubMed
UniProtP9WPL5|CP142_MYCTU Steroid C26-monooxygenase (Gene Name=cyp142)

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