Structure of PDB 7zjw Chain E

Receptor sequence

>7zjwE (length=519) Species: 9606 (Homo sapiens)

GRRVNVNVGVLGHIDSGKTALARALSTLDLGFSCFSVPLPARLRSSLPGE
PLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVI
GQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIP
VAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVDHCFS
IKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQG
DRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKA
KFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKD
HCPREQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGILLHGL
EDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFV
GLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPASEPSQHV
VLSLTFKRYVFDTHKRMVQ

3D structure

• PDB: 7zjw Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon.
• Chain: E
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 3.6.5.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	S98 H230 F232 V240 Q271 C289 E299 R300 H336 L433 D434 A435 K471 E497 N499	S65 H197 F199 V207 Q238 C256 E266 R267 H303 L379 D380 A381 K417 E443 N445
BS02	rna	E	R491 F522 Q524 K527	R437 F468 Q470 K473
BS03	rna	E	I16 P191 I437	I14 P158 I383
BS04	rna	E	G236 Q292 F522 Q524	G203 Q259 F468 Q470
BS05	GTP	E	D17 G19 K20 T21 A22 N146 D149 A185 A186 K187 P188	D15 G17 K18 T19 A20 N113 D116 A152 A153 K154 P155

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0035368 selenocysteine insertion sequence binding
• GO:0043021 ribonucleoprotein complex binding

Biological Process

• GO:0001514 selenocysteine incorporation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zjw, PDBe:7zjw, PDBj:7zjw
• PDBsum: 7zjw
• PubMed: 35709277
• UniProt: P57772|SELB_HUMAN Selenocysteine-specific elongation factor (Gene Name=EEFSEC)