Structure of PDB 7xyl Chain E

Receptor sequence

>7xylE (length=482) Species: 285500 (Streptomyces luteocolor)

QTDVIVVGNGVLGLSVGVEIARTRPDVRVTLLGKPARQYGATPAAGAMLG
AFGEVTAHALASEHGRKKHALAVQAQRLWPEWIESLEATGTAADGRIKTA
DDTVVLLNTVGHSALDDANFAAVLTALKEANAPHEEIAVESVDWIDPDPN
SRPLRALHIEGEGSVDSGILLAALERSFLQAGGRLHPVDATEIRASHGRV
EGVVTDDGDFLPAGHVVVAAGARSQRLVAALPGLAHRIPRIYDGVGVSAL
VDTWDGSGPATVLRTSNRAFACGLHLVPRAGGSVYIGATNAVCLEPRGAA
SIEETVFLFNCATHQLHRGLNGSELRKVQVGSRPAPIDGFPLIGGTSVEG
LWMLSGTYRDGLHMSPLLARHVVSLMDGGTGVDGLREFRPERDLISAWSR
EEILDDVVRHTMATGYQFPWRLPLEWPHMMETFLQGPFAELADRLSDTYT
PPADLMTAIMFSEREQQDELIAYYADVHREWH

3D structure

• PDB: 7xyl N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry.
• Chain: E
• Resolution: 2.099 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	E	G17 N18 G19 V20 L21 G42 R46 Q47 G49 A50 T51 A54 M57 D198 A199 A229 G230 R232 G340 R342 P343 T366 Y367 R368 D369 G370 L371 H372	G8 N9 G10 V11 L12 G33 R37 Q38 G40 A41 T42 A45 M48 D189 A190 A220 G221 R223 G331 R333 P334 T357 Y358 R359 D360 G361 L362 H363
BS02	GLY	E	R342 R368	R333 R359
BS03	I55	E	N299 R306 S310 E312 T423 T466 F470	N290 R297 S301 E303 T414 T457 F461

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7xyl, PDBe:7xyl, PDBj:7xyl
• PDBsum: 7xyl
• PubMed: 37137912
• UniProt: A0A125SZC1