Structure of PDB 7tys Chain E

Receptor sequence

>7tysE (length=1431) Species: 10034 (Cricetus cricetus)

MPLAFCGTENHSAAYRVDQGVLNNGCFVDALNVVPHVFLLFITFPILFIG
WGSQSSKVHIHHSTWLHFPGHNLRWILTFILLFVLVCEIAEGILSDGVTE
SRHLHLYMPAGMAFMAAITSVVYYHNIETSNFPKLLIALLIYWTLAFITK
TIKFVKFYDHAIGFSQLRFCLTGLLVILYGMLLLVEVNVIRVRRYIFFKT
PREVKPPEDLQDLGVRFLQPFVNLLSKGTYWWMNAFIKTAHKKPIDLRAI
AKLPIAMRALTNYQRLCVAFDAQARKDTQSPQGARAIWRALCHAFGRRLI
LSSTFRILADLLGFAGPLCIFGIVDHLGKENHVFQPKTQFLGVYFVSSQE
FLGNAYVLAVLLFLALLLQRTFLQASYYVAIETGINLRGAIQTKIYNKIM
HMSTSNLSMGEMTAGQICNLVAIDTNQLMWFFFLCPNLWTMPVQIIVGVI
LLYYILGVSALIGAAVIILLAPVQYFVATKLSQAQRTTLEHSNERLKQTN
EMLRGMKLLKLYAWESIFCSRVEVTRRKEMTSLRAFAVYTSISIFMNTAI
PIAAVLITFVGHVSFFKESDLSPSVAFASLSLFHILVTPLFLLSSVVRST
VKALVSVQKLSEFLSSAEIREEQCNFCVQIIGGFFTWTPDGIPTLSNITI
RIPRGQLTMIVGQVGCGKSSLLLATLGEMQKVSGAVFWNSNSRGPVAYAS
QKPWLLNATVEENITFESPFNKQRYKMVIEACSLQPDIDILPHGDQTQIG
ERGINLSGGQRQRISVARALYQQTNVVFLDDPFSALDVHLSDHLMQAGIL
ELLRDDKRTVVLVTHKLQYLPHADWIIAMKDGTIQREGTLKDFQRSECQL
FEHWKSSVLHQRAKIPWRACTKYLSSAGILLLSLLVFSQLLKHMVLVAID
YWLAKWTDSALVDQSVYAMVFTLLCSLGIVLCLVTSVTVEWTGLKVAKRL
HRSLLNRIILAPMRFFETTPLGSILNRFSSDCNTIDQHIPSTLECLSRST
LLCVSALTVISYVTPVFLVALLPLAVVCYFIQKYFRVASRDLQQLDDTTQ
LPLVSHFAETVEGLTTIRAFRYEARFQQKLLEYTDSNNIASLFLTAANRW
LEVCMEYIGACVVLIAAATSISNSLHRELSAGLVGLGLTYALMVSNYLNW
MVRNLADMEIQLGAVKRIHALLKTEAESYEGLLAPSLIPKNWPDQGKIQI
QNLSVRYDSSLKPVLKHVNTLISPGQKIGICGRTGSGKSSFSLAFFRMVD
MFEGRIIIDGIDIAKLPLHTLRSRLSIILQDPVLFSGTIRFNLDPEKKCS
DSTLWEALEIAQLKLVVKALPGGLDAIITEGGENFSQGQRQLFCLARAFV
RKTSIFIMDEATASIDMATENILQKVVMTAFADRTVVTIAHRVHTILSAD
LVMVLKRGAILEFDKPETLLSQKDSVFASFV

3D structure

• PDB: 7tys Ligand-mediated Structural Dynamics of a Mammalian Pancreatic K ATP Channel.
• Chain: E
• Resolution: 3.41 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	P5S	E	I46 F132 K134 A138 I141	I46 F132 K134 A138 I141
BS02	65I	E	G20 V21	G20 V21
BS03	BJX	E	Y377 I381 L434 N437 L592 N1245 R1246 W1297 R1300	Y377 I381 L434 N437 L592 N1098 R1099 W1150 R1153
BS04	ATP	E	N406 W688 V715 G716 C717 G718 K719 S720 S721 Q775	N406 W637 V664 G665 C666 G667 K668 S669 S670 Q701
BS05	65I	E	W65 N223 L225 S226 W231	W65 N223 L225 S226 W231
BS06	65I	E	I89 A90 I93 G97 F114 Y356	I89 A90 I93 G97 F114 Y356
BS07	65I	E	W231 F1177 Y1181	W231 F1030 Y1034
BS08	P5S	E	F79 I80 F83 L224 K227 G228 F305 L368 T371 F372	F79 I80 F83 L224 K227 G228 F305 L368 T371 F372
BS09	65I	E	F83 A315 L318 F321 L364 A365 L451	F83 A315 L318 F321 L364 A365 L451
BS10	65I	E	L47 W51 L82 H125 N126 T129	L47 W51 L82 H125 N126 T129

Gene Ontology

Molecular Function

• GO:0005267 potassium channel activity
• GO:0005524 ATP binding
• GO:0008281 sulfonylurea receptor activity
• GO:0016887 ATP hydrolysis activity
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0006813 potassium ion transport
• GO:0055085 transmembrane transport
• GO:0071805 potassium ion transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0032991 protein-containing complex

External links

• PDB: RCSB:7tys, PDBe:7tys, PDBj:7tys
• PDBsum: 7tys
• PubMed: 35964676
• UniProt: Q09427|ABCC8_CRICR ATP-binding cassette sub-family C member 8 (Gene Name=ABCC8)