Structure of PDB 7t20 Chain E

Receptor sequence
>7t20E (length=440) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
KVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMAR
LQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYAD
IVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRAN
KDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPS
DLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLA
SLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVR
SRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKE
LNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVY
HENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYA
3D structure
PDB7t20 Sequence of conformation changes of DnaB helicase during DNA unwinding and priming in Escherichia coli
ChainE
Resolution4.7 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna E N386 R387 E404 N362 R363 E380
BS02 ANP E P233 S234 G236 K237 T238 T239 E262 R271 Q281 T282 G455 Q456 P209 S210 G212 K213 T214 T215 E238 R247 Q257 T258 G431 Q432
BS03 MG E T238 E262 T214 E238
BS04 ANP E G444 P445 G420 P421
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016853 isomerase activity
GO:0016887 ATP hydrolysis activity
GO:0042802 identical protein binding
GO:0043139 5'-3' DNA helicase activity
Biological Process
GO:0006260 DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006269 DNA replication, synthesis of primer
GO:0006270 DNA replication initiation
GO:0010212 response to ionizing radiation
GO:0031297 replication fork processing
GO:0032508 DNA duplex unwinding
Cellular Component
GO:0005829 cytosol
GO:0030894 replisome
GO:0033202 DNA helicase complex
GO:1990077 primosome complex
GO:1990100 DnaB-DnaC complex
GO:1990156 DnaB-DnaG complex
GO:1990158 DnaB-DnaC-DnaT-PriA-PriB complex
GO:1990159 DnaB-DnaC-DnaT-PriA-PriC complex
GO:1990160 DnaB-DnaC-Rep-PriC complex

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Molecular Function
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Cellular Component
External links
PDB RCSB:7t20, PDBe:7t20, PDBj:7t20
PDBsum7t20
PubMed
UniProtP0ACB0|DNAB_ECOLI Replicative DNA helicase DnaB (Gene Name=dnaB)

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