Structure of PDB 7s5x Chain E

Receptor sequence

>7s5xE (length=1412) Species: 9606 (Homo sapiens)

MGPLAFCGSENHSAAYRVDQGVLNNGCFVDALNVVPHVFLLFITFPILFI
GWGSQSSKVHIHHSTWLHFPGHNLRWILTFMLLFVLVCEIAEGILSDGVT
ESHHLHLYMPAGMAFMAAVTSVVYYHNIETSNFPKLLIALLVYWTLAFIT
KTIKFVKFLDHAIGFSQLRFCLTGLLVILYGMLLLVEVNVIRVRRYIFFK
TPREVKPPEDLQDLGVRFLQPFVNLLSKGTYWWMNAFIKTAHKKPIDLRA
IGKLPIAMRALTNYQRLCEAFDAQVGARAIWQALSHAFGRRLVLSSTFRI
LADLLGFAGPLCIFGIVDHLGKLGVYFVSSQEFLANAYVLAVLLFLALLL
QRTFLQASYYVAIETGINLRGAIQTKIYNKIMHLSTSNLSMGEMTAGQIC
NLVAIDTNQLMWFFFLCPNLWAMPVQIIVGVILLYYILGVSALIGAAVII
LLAPVQYFVATKLSQAQRSTLEYSNERLKQTNEMLRGIKLLKLYAWENIF
RTRVETTRRKEMTSLRAFAIYTSISIFMNTAIPIAAVLITFVGHVSFFKE
ADFSPSVAFASLSLFHILVTPLFLLSSVVRSTVKALVSVQKLSEFLSSAE
IRDNCCVQIMGGYFTWTPDGIPTLSNITIRIPRGQLTMIVGQVGCGKSSL
LLAALGEMQKVSGAVFWSRGPVAYASQKPWLLNATVEENIIFESPFNKQR
YKMVIEACSLQPDIDILPHGDQTQIGERGINLSGGQRQRISVARALYQHA
NVVFLDDPFSALDIHLSDHLMQAGILELLRDDKRTVVLVTHKLQYLPHAD
WIIAMKDGTIQREGTLKDFQRSECQLFEHWKTLMNRQDQELEKEPWRACA
KYLSSAGILLLSLLVFSQLLKHMVLVAIDYWLAKWTDSADQTVYAMVFTV
LCSLGIVLCLVTSVTVEWTGLKVAKRLHRSLLNRIILAPMRFFETTPLGS
ILNRFSSDCNTIDQHIPSTLECLSRSTLLCVSALAVISYVTPVFLVALLP
LAIVCYFIQKYFRVASRDLQQLDDTTQLPLLSHFAETVEGLTTIRAFRYE
ARFQQKLLEYTDSNNIASLFLTAANRWLEVRMEYIGACVVLIAAVTSISN
SLHRELSAGLVGLGLTYALMVSNYLNWMVRNLADMELQLGAVKRIHGLLK
TEAESYEGLLAPSLIPKNWPDQGKIQIQNLSVRYDSSLKPVLKHVNALIA
PGQKIGICGRTGSGKSSFSLAFFRMVDTFEGHIIIDGIDIAKLPLHTLRS
RLSIILQDPVLFSGTIRFNLDPERKCSDSTLWEALEIAQLKLVVKALPGG
LDAIITEGGENFSQGQRQLFCLARAFVRKTSIFIMDEATASIDMATENIL
QKVVMTAFADRTVVTIAHRVHTILSADLVIVLKRGAILEFDKPEKLLSRK
DSVFASFVRADK

3D structure

• PDB: 7s5x Molecular structure of an open human K ATP channel.
• Chain: E
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	E	R1111 Y1354 V1361 T1381 G1382 S1383 G1384 K1385 S1386 S1387	R941 Y1184 V1191 T1211 G1212 S1213 G1214 K1215 S1216 S1217
BS02	MG	E	S721 Q775	S648 Q677
BS03	MG	E	K1385 S1386	K1215 S1216
BS04	ATP	E	W689 G717 G719 K720 S721 S722 Q775 E1480 N1481 S1483 G1485 Q1486	W616 G644 G646 K647 S648 S649 Q677 E1310 N1311 S1313 G1315 Q1316

Gene Ontology

Molecular Function

• GO:0005267 potassium channel activity
• GO:0005524 ATP binding
• GO:0008281 sulfonylurea receptor activity
• GO:0015272 ATP-activated inward rectifier potassium channel activity
• GO:0016887 ATP hydrolysis activity
• GO:0019829 ATPase-coupled monoatomic cation transmembrane transporter activity
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0043531 ADP binding
• GO:0044325 transmembrane transporter binding
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0001508 action potential
• GO:0001678 intracellular glucose homeostasis
• GO:0006813 potassium ion transport
• GO:0007565 female pregnancy
• GO:0007613 memory
• GO:0008542 visual learning
• GO:0009268 response to pH
• GO:0009410 response to xenobiotic stimulus
• GO:0010043 response to zinc ion
• GO:0010989 negative regulation of low-density lipoprotein particle clearance
• GO:0016525 negative regulation of angiogenesis
• GO:0031669 cellular response to nutrient levels
• GO:0032496 response to lipopolysaccharide
• GO:0032760 positive regulation of tumor necrosis factor production
• GO:0032868 response to insulin
• GO:0035774 positive regulation of insulin secretion involved in cellular response to glucose stimulus
• GO:0043268 positive regulation of potassium ion transport
• GO:0046676 negative regulation of insulin secretion
• GO:0050768 negative regulation of neurogenesis
• GO:0050905 neuromuscular process
• GO:0055085 transmembrane transport
• GO:0060253 negative regulation of glial cell proliferation
• GO:0061535 glutamate secretion, neurotransmission
• GO:0061855 negative regulation of neuroblast migration
• GO:0071805 potassium ion transmembrane transport
• GO:0098662 inorganic cation transmembrane transport
• GO:1900721 positive regulation of uterine smooth muscle relaxation
• GO:1905075 positive regulation of tight junction disassembly
• GO:1905604 negative regulation of blood-brain barrier permeability
• GO:1990573 potassium ion import across plasma membrane

Cellular Component

• GO:0005886 plasma membrane
• GO:0008282 inward rectifying potassium channel
• GO:0016020 membrane
• GO:0030672 synaptic vesicle membrane
• GO:0031004 potassium ion-transporting ATPase complex
• GO:0032991 protein-containing complex
• GO:0042383 sarcolemma
• GO:0042734 presynaptic membrane

External links

• PDB: RCSB:7s5x, PDBe:7s5x, PDBj:7s5x
• PDBsum: 7s5x
• PubMed: 34815345
• UniProt: Q09428|ABCC8_HUMAN ATP-binding cassette sub-family C member 8 (Gene Name=ABCC8)