Structure of PDB 7rfi Chain E

Receptor sequence

>7rfiE (length=422) Species: 9606 (Homo sapiens)

GHETAAQRYSARLLQAGYMADYLISGGTGYVPEDGLTAQQLFASADGLTY
NDFLILPGFIDFIADEVDLTSALTRKITLKTPLISSPMDTVTEADMAIAM
ALMGGIGFIHHNCTPEFQANEVRKVKKFEQGFITDPTLKEANAIIARTDL
KKNRDYPLASKDSQKQLLCGAAVGTREDDKYRLDLLTQAGVDVIVLDSSQ
GNSVYQIAMVHYIKQKYPHLQVIGGNVVTAAQAKNLIDAGVDGLRVGMGC
GSICITQEVMACGRPQGTAVYKVAEYARRFGVPIIADGGIQTVGHVVKAL
ALGASTVMMGSLLAATTEAPGEYFFSDGVRLKKYRGMGSLDAVKIAQGVS
GSIQDKGSIQKFVPYLIAGIQHGCQDIGARSLSVLRSMMYSGELKFEKRT
MSAQIEGGVHGLHSYTFLPFTK

3D structure

• PDB: 7rfi IMPDH1 retinal variants control filament architecture to tune allosteric regulation.
• Chain: E
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 1.1.1.205: IMP dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GTP	E	K109 Q112 P118 T225 D226	K127 Q130 P136 T148 D149
BS02	GTP	E	E111 Q112 L227 N230 K238 K242	E129 Q130 L150 N153 K161 K165
BS03	IMP	E	S68 M70 R322 G328 S329 I330 C331 D364 G365 G387 S388 Y411 G413 M414 G415 Q441	S86 M88 R245 G251 S252 I253 C254 D287 G288 G310 S311 Y334 G336 M337 G338 Q347
BS04	NAD	E	T252 R253 D274 S276 Y282 N303 T333 M414	T175 R176 D197 S199 Y205 N226 T256 M337
BS05	NAD	E	I45 Q469	I63 Q375

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0003938 IMP dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006164 purine nucleotide biosynthetic process
• GO:0006177 GMP biosynthetic process

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm

External links

• PDB: RCSB:7rfi, PDBe:7rfi, PDBj:7rfi
• PDBsum: 7rfi
• PubMed: 35013599
• UniProt: Q5H9Q6