Structure of PDB 7mi5 Chain E

Receptor sequence

>7mi5E (length=95) Species: 243231 (Geobacter sulfurreducens PCA)

MEHLYIVSYDIRNQRRWRRLFKTMHGFGCWLQLSVFQCRLDRIRIIKMEA
AINEIVNHAEDHVLILDLGPAENVKPKVSSIGKTFDPILRQAVIV

3D structure

• PDB: 7mi5 Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
• Chain: E
• Resolution: 3.57 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	R18 L33	R18 L33
BS02	dna	E	Y9 D10 I11 R12 W17 F21 L33 S34	Y9 D10 I11 R12 W17 F21 L33 S34
BS03	MN	E	Y9 D10 S34	Y9 D10 S34

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0008150 biological_process
• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7mi5, PDBe:7mi5, PDBj:7mi5
• PDBsum: 7mi5
• PubMed: 34588691
• UniProt: Q74H35|CAS2_GEOSL CRISPR-associated endoribonuclease Cas2 (Gene Name=cas2)