Structure of PDB 7fsc Chain E

Receptor sequence

>7fscE (length=419) Species: 9606 (Homo sapiens)

AFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVER
LKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAI
ALDTKGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEG
HGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKM
MIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLS
GETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVT
AIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQ
VHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVV
TGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7fsc Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: E
• Resolution: 1.855 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	E	L443 T444 T445 T446 S449 W494 R501 G526 R528 G530 S531 G532 Y533 T534	L319 T320 T321 T322 S325 W370 R377 G402 R404 G406 S407 G408 Y409 T410
BS02	OXL	E	K282 E284 A305 G307 D308 T340	K158 E160 A181 G183 D184 T216
BS03	MG	E	E284 D308	E160 D184
BS04	ODL	E	F38 L39 L42 Y402 Q405 L406 E409	F16 L17 L20 Y278 Q281 L282 E285

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7fsc, PDBe:7fsc, PDBj:7fsc
• PDBsum: 7fsc
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)