Structure of PDB 7fs5 Chain E

Receptor sequence

>7fs5E (length=418) Species: 9606 (Homo sapiens)

AFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVER
LKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAI
ALDTKGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEG
HGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKM
MIGRCNLAGKPVVCATQMLSMITKPRPTRAETSDVANAVLDGADCIMLSG
ETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTA
IGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQV
HLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVT
GWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7fs5 Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: E
• Resolution: 2.182 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	E	L443 T444 T445 T446 S449 W494 R501 G526 R528 G530 S531 Y533 T534	L318 T319 T320 T321 S324 W369 R376 G401 R403 G405 S406 Y408 T409
BS02	OXL	E	K282 E284 A305 G307 D308 T340	K158 E160 A181 G183 D184 T216
BS03	MG	E	E284 D308	E160 D184
BS04	O8O	E	F38 L39 L365 Y402 Q405 L406 E409	F16 L17 L240 Y277 Q280 L281 E284

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7fs5, PDBe:7fs5, PDBj:7fs5
• PDBsum: 7fs5
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)