Structure of PDB 7frx Chain E

Receptor sequence

>7frxE (length=419) Species: 9606 (Homo sapiens)

AFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVER
LKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAI
ALDTKGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEG
HGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKM
MIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLS
GETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVT
AIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQ
VHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVV
TGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7frx Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: E
• Resolution: 1.847 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	E	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 Y533 T534	L319 T320 T321 T322 S325 W370 R377 G402 R404 P405 G406 S407 Y409 T410
BS02	OXL	E	K282 E284 A305 G307 D308 T340	K158 E160 A181 G183 D184 T216
BS03	MG	E	E284 D308	E160 D184
BS04	O88	E	F38 L39 Y402 Q405 L406 E409	F16 L17 Y278 Q281 L282 E285

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7frx, PDBe:7frx, PDBj:7frx
• PDBsum: 7frx
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)