Structure of PDB 7egq Chain E

Receptor sequence

>7egqE (length=588) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

VGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCN
APGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSD
NVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGI
ATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFE
KGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITG
LYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPS
ARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPAVECFDKFKVNSTLEQ
YVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQ
LPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSA
LVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNP
AWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAH
SCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEI

3D structure

• PDB: 7egq Coupling of N7-methyltransferase and 3'-5' exoribonuclease with SARS-CoV-2 polymerase reveals mechanisms for capping and proofreading.
• Chain: E
• Resolution: 3.35 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	E	C16 C19 H33 H39	C15 C18 H32 H38
BS02	ZN	E	C8 C26 C29	C7 C25 C28
BS03	ZN	E	C50 C55 C72 H75	C49 C54 C71 H74

Gene Ontology

Molecular Function

• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0008270 zinc ion binding

External links

• PDB: RCSB:7egq, PDBe:7egq, PDBj:7egq
• PDBsum: 7egq
• PubMed: 34143953
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)