Structure of PDB 7cse Chain E

Receptor sequence

>7cseE (length=309) Species: 3702 (Arabidopsis thaliana)

KTRVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFK
KLGARIVEGSFSDHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEA
IKEAGNVKRFLPSEFGMDPPRMGHALPPGRETFDQKMEVRQAIEAAGIPY
TYVVGACFAAYFAGNLSQMVTLLPPKEKVNIYGDGNVKVVFADEDDIAKY
TAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKELEKTNIAAQD
FLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGEDEEASSLYPDVKYKR
MDDYLRMFL

3D structure

• PDB: 7cse Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases.
• Chain: E
• Resolution: 2.44019 Å

Enzymatic activity

• Enzyme Commision number: 1.23.1.1: (+)-pinoresinol reductase.
  1.23.1.3: (-)-pinoresinol reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GFR	E	F94 G124 M125 Y169 F170 M177 H276	F86 G116 M117 Y161 F162 M169 H268
BS02	NDP	E	G16 T18 G19 Y20 I21 R41 K50 F69 A88 M89 S90 G91 V92 R95 N98 S121 E122 F123 K144 C165 F166 F170	G8 T10 G11 Y12 I13 R33 K42 F61 A80 M81 S82 G83 V84 R87 N90 S113 E114 F115 K136 C157 F158 F162

Gene Ontology

Molecular Function

• GO:0010283 pinoresinol reductase activity
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0009807 lignan biosynthetic process

External links

• PDB: RCSB:7cse, PDBe:7cse, PDBj:7cse
• PDBsum: 7cse
• PubMed: 33990581
• UniProt: Q9FVQ6|PILR1_ARATH Pinoresinol reductase 1 (Gene Name=PRR1)