Structure of PDB 7acj Chain E

Receptor sequence

>7acjE (length=177) Species: 83333 (Escherichia coli K-12)

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

• PDB: 7acj Structures of tmRNA and SmpB as they transit through the ribosome.
• Chain: E
• Resolution: 3.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	N37 G39 G41 E42 T68 R71 K72 V74 G76 F77 I85 K88 S121 F122 D123 R125 N127 S129 G131 V132 G151 L152 D153 T155 T157	N36 G38 G40 E41 T67 R70 K71 V73 G75 F76 I84 K87 S120 F121 D122 R124 N126 S128 G130 V131 G150 L151 D152 T154 T156
BS02	rna	E	S24 M26 Q27 Q63 K64 L66 T90 R92	S23 M25 Q26 Q62 K63 L65 T89 R91
BS03	rna	E	S73 A75 K78 R80	S72 A74 K77 R79

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7acj, PDBe:7acj, PDBj:7acj
• PDBsum: 7acj
• PubMed: 34389707
• UniProt: P62399|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)