Structure of PDB 6vz8 Chain E

Receptor sequence

>6vz8E (length=541) Species: 3702 (Arabidopsis thaliana)

FISRFAPDQPRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSSI
RNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSGLADALLDS
VPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRIIE
EAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMPKPPED
SHLEQIVRLISKKPVLYVGGGCLNSSDELGRFVELTGIPVASTLMGLGSY
PCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRVTGIVHIDIDS
AEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKLDFGVWRNELNVQK
QKFPLSFFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAAQFYNYKK
PRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELAT
IRVENLPVKVLLLNNQHLGMVMQWEDRFYKANRAHTFLGDPAQEIFPNML
LFAAACGIPAARVTKKADLREAIQTMLDTPGPYLLDVICPH

3D structure

• PDB: 6vz8 Structures of fungal and plant acetohydroxyacid synthases.
• Chain: E
• Resolution: 3.45 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y118 G120 G121 A122 S123 E144 T167 F206 Q207 E208 K256 M351 V378 V485 G511 M513 D538 N565 H567 L568 M570 V571 W574
• Catalytic site (residue number reindexed from 1): Y32 G34 G35 A36 S37 E58 T81 F120 Q121 E122 K170 M263 V290 V385 G411 M413 D438 N465 H467 L468 M470 V471 W474
• Enzyme Commision number: 2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TPP	E	E144 P170	E58 P84
BS02	FAD	E	G307 G309 T331 L332 L349 R373 R377 D395 I396 D414 V415 G509	G219 G221 T243 L244 L261 R285 R289 D297 I298 D316 V317 G409
BS03	TPP	E	V485 G486 Q487 H488 M513 G537 G539 S540 L568 G569 M570 V571	V385 G386 Q387 H388 M413 G437 G439 S440 L468 G469 M470 V471

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0003984 acetolactate synthase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0009082 branched-chain amino acid biosynthetic process

External links

• PDB: RCSB:6vz8, PDBe:6vz8, PDBj:6vz8
• PDBsum: 6vz8
• PubMed: 32640464
• UniProt: P17597|ILVB_ARATH Acetolactate synthase, chloroplastic (Gene Name=ALS)